PXD014210-1
PXD014210 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis reveals that topoisomerase 2A is associated with defective sperm head morphology |
| Description | Male infertility is widespread and estimated to affect 1 in 20 men. Whilst in some cases the aetiology of the condition is well understood, for at least 50% of men, the underlying cause is yet to be classified. The majority of infertile men produce enough spermatozoa, however it is a combination of poor sperm motility, associated with a modified cell morphology that contribute to infertility. One of the major limitations of using morphology to inform male fertility status is that the evaluation is highly subjective, due to the fact that it has to be done by microscopic examination. As such, biomarkers of male infertility are needed to help establish a more consistent diagnosis. In the present study, we compared nuclear extracts from both high- and low-quality spermatozoa by LC-MS/MS based proteomic analysis. Our data shows that nuclear retention of proteins is a common facet amongst low quality male reproductive cells. In particular, we demonstrate that the presence of Topoisomerase 2A in sperm heads, is highly correlated to poor head morphology. Topoisomerase A is therefore a potential new biomarker for confirming male infertility in clinical practice. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-01-05 |
| AnnouncementXML | Submission_2020-01-05_15:59:23.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jacob Netherton |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-06-10 21:13:46 | ID requested | |
| ⏵ 1 | 2020-01-05 15:59:24 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Spermatozoa, Male Reproduction, SWATH |
Contact List
| Jacob K Netherton | |
|---|---|
| contact affiliation | University of Newcastle |
| contact email | jacob.netherton@uon.edu.au |
| lab head | |
| Mark A Baker | |
| contact affiliation | University of Newcastle |
| contact email | mark.baker@newcastle.edu.au |
| lab head | |
| Jacob Netherton | |
| contact affiliation | University of Newcastle |
| contact email | jacob.netherton@uon.edu.au |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083954/ |
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