PXD014187 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Effect of Nop53 depletion on the exosome interactome |
Description | In order to evaluate how Nop53 modulates the interaction of Rrp6 and Rrp43 with other factors, a coimmunoprecipitation assay was performed both in the presence (-doxicycline) and upon depletion (+ doxicycline) of Nop53 using the condional strain Δnop53/tetOff::GFP-NOP53 carrying the Rrp6-TAP or Rrp43-TAP fusion. As a negative control, the same strain carrying only the TAP tag was employed. For each condition, the elution of biological triplicates was subjected to label free quantitative analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-08 |
AnnouncementXML | Submission_2021-09-08_11:15:11.783.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Felipe Bagatelli |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-10 01:24:10 | ID requested | |
⏵ 1 | 2021-09-08 11:15:12 | announced | |
Publication List
Cepeda LPP, Bagatelli FFM, Santos RM, Santos MDM, Nogueira FCS, Oliveira CC, The ribosome assembly factor Nop53 controls association of the RNA exosome with pre-60S particles in yeast. J Biol Chem, 294(50):19365-19380(2019) [pubmed] |
Keyword List
submitter keyword: Nop53, Ribosome biogenesis, RNA exosome, rRNA processing |
Contact List
Carla C. Oliveira |
contact affiliation | Department of Biochemistry, Institute of Chemistry, University of São Paulo,São Paulo, SP, Brazil |
contact email | ccoliv@iq.usp.br |
lab head | |
Felipe Bagatelli |
contact affiliation | Univestity of Sao Paulo |
contact email | felipe.bagatelli@usp.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014187
- Label: PRIDE project
- Name: Effect of Nop53 depletion on the exosome interactome