PXD014174 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Beta-glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus |
Description | A clone encoding carboxymethylcellulase activity was isolated during functional screening of a human gut metagenomic library using Lactococcus lactis MG1363 as heterologous host. The insert sequence revealed a glycoside hydrolase family 9 (GH9) catalytic domain with sequence similarity to a gene from Coprococcus sp. ART55/1, which is closely related to Coprocococcus eutactus. Surveys of available genomes indicated a limited distribution of GH9 domains among dominant human colonic anaerobes. Genomes of two Coprococcus-related strains showed the presence of two GH9-encoding and four GH5-encoding genes, however, the strains did not appear to be able to degrade cellulose. Instead, they grew well on beta-glucans and one of the strains also showed growth on galactomannan, galactan and glucomannan. Gene expression and proteomic analysis of Coprococcus sp. ART55/1 grown on cellobiose, beta-glucan and lichenan led to a similar change in expression in comparison to glucose. On beta-glucan and lichenan only, one of the four GH5 genes was strongly upregulated. Growth on glucomannan lead to a transcriptional response of many genes, in particular a strong upregulation of glycoside hydrolases involved in mannan degradation. Thus, beta-glucans are a major growth substrate for species related to Coprococcus eutactus, with glucomannan and galactans alternative substrates for some strains. |
HostingRepository | PRIDE |
AnnounceDate | 2020-03-10 |
AnnouncementXML | Submission_2020-03-10_04:14:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sophie Shaw |
SpeciesList | scientific name: Coprococcus sp. ART55/1; NCBI TaxID: 751585; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-07 03:52:59 | ID requested | |
⏵ 1 | 2020-03-10 04:14:45 | announced | |
Publication List
Alessi AM, Gray V, Farquharson FM, Flores-L, รณ, pez A, Shaw S, Stead D, Wegmann U, Shearman C, Gasson M, Collie-Duguid ESR, Flint HJ, Louis P, -Glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus. Environ Microbiol, 22(6):2150-2164(2020) [pubmed] |
Keyword List
submitter keyword: Coprococcus eutactus |
beta-glucan; |
Contact List
Petra Louis |
contact affiliation | Rowett Institute Gut Health Theme University of Aberdeen Foresterhill Aberdeen AB25 2ZD |
contact email | p.louis@abdn.ac.uk |
lab head | |
Sophie Shaw |
contact affiliation | University of Aberdeen |
contact email | s.shaw@abdn.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014174
- Label: PRIDE project
- Name: Beta-glucan is a major growth substrate for human gut bacteria related to Coprococcus eutactus