PXD014153 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus |
Description | Daphnia infochemicals can induce defensive responses in microalgae Scenedesmus spp., including colony formation and flocculation. We investigated the phenomenon using a combination of microalgal biology, physiological ecology, and quantitative isobaric tagging (iTRAQ) proteomics. We have discovered that flocculation as a defense response occurs at the early ‘alarm’ phase and requires increased energy resources, with an important role proposed in cysteine synthesis. It appears to be initially stimulated by the production of an extracellular matrix (mainly composed ofby polysaccharides and fatty acids), and later sustained at the ‘acclimation’ stage through Mitogen-activated protein kinase (MAPK) signalling cascades. Defensive strategies leading to colony formation required investment into fatty acids metabolism, to ensure the accurate separation of membranes during cell division. Furthermore, higher energy demands were required at the alarm phase which subsequently decreased at the acclimation stage, thus suggesting a trade-off between colony formation and support of floc form. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-06 |
AnnouncementXML | Submission_2020-06-01_14:48:55.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sebastiana Roccuzzo |
SpeciesList | scientific name: Desmodesmus subspicatus; NCBI TaxID: 104105; |
ModificationList | methylthiolated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-06 02:28:54 | ID requested | |
1 | 2020-05-06 01:29:30 | announced | |
⏵ 2 | 2020-06-01 14:48:56 | announced | 2020-06-01: Updated publication reference for PubMed record(s): 32457714. |
Publication List
Roccuzzo S, Couto N, Karunakaran E, Kapoore RV, Butler TO, Mukherjee J, Hansson EM, Beckerman AP, Pandhal J, Unraveled by Quantitative Proteomics. Front Microbiol, 11():792(2020) [pubmed] |
Keyword List
submitter keyword: Daphnia infochemicals, Scenedesmus, induced defences, predation, flocculation, colony formation iTRAQ proteomics |
Contact List
Jagroop Pandhal |
contact affiliation | Department of Chemical and Biological Engineering University of Sheffield Mappin Street, Sheffield S1 3JD United Kingdom |
contact email | j.pandhal@sheffield.ac.uk |
lab head | |
Sebastiana Roccuzzo |
contact affiliation | Faculty of Environment, J. E. Purkyne University in Usti-nad-Labem |
contact email | drnellaroccuzzo@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014153
- Label: PRIDE project
- Name: The mechanism of infochemicals induced colony formation and flocculation in the microalga Scenedesmus subspicatus