PXD014116-1

PXD014116 is an original dataset announced via ProteomeXchange.

Title	Protein phosphorylation and N-glycosylation in the early stage of Spiroplasma eriocheiris infect Eriocheir sinensis
Description	As a novel lethal pathogen of Eriocheirsinensistremor disease,Spiroplasma eriocheiris, has led into catastrophic economic losses in aquaculture.The hemocytes of E. sinensis is the first target cells of S. eriocheiris. Our study is the first time designed to understanding of the phosphoproteome and N-glycoproteome dynamics of E. sinensis hemocytesunderS. eriocheiris infected at the early phage. In the current study, the phosphoproteome and N-glycoproteome changes of E. sinensishemocytesafter S. eriocheiris infection were obtained usingtandem mass tags(TMT) labeling and affinity enrichment followed by high-resolution Liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) analysis. Using a 1.2-fold change in expression as a physiologically significant benchmark.In total, 549 differentially expressed phosphoproteins were reliably quantified, including 278 up-regulated phosphoproteinsand 271 down-regulated phosphoproteins; 167 differentially expressed N-glycosylated proteins were reliably quantified, including 79 up-regulated glycosylated proteins and 88 down-regulated glycosylated proteins subsequented to S. eriocheiris infection.Gene ontology (GO) annotation, protein domain annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation, subcellular localization annotation and protein-protein interaction network were used to analysis those significantly differentlyexpression proteins shown that many biological process and pathway are participate inS. eriocheiris infected host cell, such as phagocytosis, ECM-receptor interaction, lysosome, prophenoloxidase system, and so on. Our study could serve as a basis to understand the relationship between E. sinensisand the pathogen S. eriocheiris, and alsoprovide reference to study protein phosphorylation and N-glycosylationin other crustaceans.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:38:17.965.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingguo Meng
SpeciesList	scientific name: Eriocheir sinensis (Chinese mitten crab); NCBI TaxID: NEWT:95602;
ModificationList	N-glycosylated residue; phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-04 03:50:56	ID requested
⏵ 1	2026-04-26 16:38:18	announced

Yao Y, Ma Y, Yin X, Yu Z, Yan J, Hou L, Xu X, Gu W, Meng Q, phosphorylation at a conserved tyrosine residue enhances ROS production to defend against Spiroplasma eriocheiris infection. Cell Commun Signal, 24(1):(2026) [pubmed]

10.1186/s12964-026-02781-8;

submitter keyword: phosphoproteome,Eriocheir sinensis,N-glycoproteome, Spiroplasma eriocheiris,hemocytes

Qingguo Meng
contact affiliation	Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences&College of Marine Science and Engineering, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China
contact email	mlzzcld@aliyun.com
lab head
Qingguo Meng
contact affiliation	Nanjing Normal University
contact email	mlzzcld@aliyun.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014116
     1. Label: PRIDE project
     2. Name: Protein phosphorylation and N-glycosylation in the early stage of Spiroplasma eriocheiris infect Eriocheir sinensis