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PXD014034-1

PXD014034 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlemTOR-regulated phosphorylation of electrogenic sodium bicarbonate cotransporter 1, NBCe1, at S255-257 regulates NBCe1 functional expression in mouse cortical astrocyte
DescriptionThe electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), is the major bicarbonate transporter expressed in astrocytes. It is highly sensitive for bicarbonate, and the main regulator of intracellular-, extracellular-, and synaptic pH, thereby modulating neuronal excitability. However, despite these essential functions, the molecular mechanisms underlying NBCe1-mediated astrocytic response to extracellular pH changes are mostly unknown. Using primary cortical astrocyte cultures we have investigated the effect of long-term extracellular metabolic alkalosis on regulation of NBCe1 using functional assays and phosphoproteomic analysis.
HostingRepositoryPRIDE
AnnounceDate2019-07-15
AnnouncementXMLSubmission_2019-07-15_04:32:21.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJana Zecha
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Q Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-28 02:09:05ID requested
12019-07-15 04:32:22announced
22024-10-22 04:55:41announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: bicarbonate, glial cells, acid-base, pH, signaling, alkalosis
Contact List
Bernhard Kuster
contact affiliationChair of Proteomics and Bioanalytics, Technical University of Munich, Germany.
contact emailkuster@tum.de
lab head
Jana Zecha
contact affiliationTUM
contact emailjana.zecha@tum.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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