PXD014034 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | mTOR-regulated phosphorylation of electrogenic sodium bicarbonate cotransporter 1, NBCe1, at S255-257 regulates NBCe1 functional expression in mouse cortical astrocyte |
Description | The electrogenic sodium bicarbonate cotransporter 1, NBCe1 (SLC4A4), is the major bicarbonate transporter expressed in astrocytes. It is highly sensitive for bicarbonate, and the main regulator of intracellular-, extracellular-, and synaptic pH, thereby modulating neuronal excitability. However, despite these essential functions, the molecular mechanisms underlying NBCe1-mediated astrocytic response to extracellular pH changes are mostly unknown. Using primary cortical astrocyte cultures we have investigated the effect of long-term extracellular metabolic alkalosis on regulation of NBCe1 using functional assays and phosphoproteomic analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2019-07-15 |
AnnouncementXML | Submission_2019-07-15_04:32:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jana Zecha |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-28 02:09:05 | ID requested | |
⏵ 1 | 2019-07-15 04:32:22 | announced | |
2 | 2024-10-22 04:55:41 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: bicarbonate, glial cells, acid-base, pH, signaling, alkalosis |
Contact List
Bernhard Kuster |
contact affiliation | Chair of Proteomics and Bioanalytics, Technical University of Munich, Germany. |
contact email | kuster@tum.de |
lab head | |
Jana Zecha |
contact affiliation | TUM |
contact email | jana.zecha@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014034
- Label: PRIDE project
- Name: mTOR-regulated phosphorylation of electrogenic sodium bicarbonate cotransporter 1, NBCe1, at S255-257 regulates NBCe1 functional expression in mouse cortical astrocyte