PXD014029-1

PXD014029 is an original dataset announced via ProteomeXchange.

Title	Assembly of a nuclear lamin coil 2 fragment
Description	Nuclear structure and function are governed by lamins, which are intermediate filaments mostly consisting of α-helices. Different lamin assembly models have been proposed based on low resolution or fragmented structures. However, their assembly mechanisms at the molecular level are poorly understood. The structure shows the anti-parallel arrangement of two coiled-coil dimers, which is important for the assembly process. We further discovered a new interaction of a coil 2 by using chemical cross-linking and mass analysis, of which the results were deposited in the PRIDE identifier PXD013144. Here we showed that a cysteine-substituted coil 2 R388C segment (286-400 amino acid residues of lamin) was dimerized by the chemical crosslinking. Our findings also provide a molecular basis for the assembly mechanisms of other intermediate filaments.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-15_04:07:09.504.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong-Hak Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-28 01:41:42	ID requested
⏵ 1	2025-12-15 04:07:10	announced

Ahn J, Jo I, Kang SM, Hong S, Kim S, Jeong S, Kim YH, Park BJ, Ha NC, Structural basis for lamin assembly at the molecular level. Nat Commun, 10(1):3757(2019) [pubmed]

10.1038/s41467-019-11684-x;

curator keyword: Biomedical

submitter keyword: Human, coil 2, lamin, nuclear protein

Yong-Hak Kim
contact affiliation	Daegu Catholic University School of Medicine
contact email	ykim@cu.ac.kr
lab head
Yong-Hak Kim
contact affiliation	Catholic University of Daegu School of Medicine
contact email	ykim@cu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD014029

PRIDE project URI

• PRIDE
  1. PXD014029
     1. Label: PRIDE project
     2. Name: Assembly of a nuclear lamin coil 2 fragment