PXD013986-3

PXD013986 is an original dataset announced via ProteomeXchange.

Title	Molecular complexity of the major urinary protein system of the Norway rat, Rattus norvegicus C4PR_LIV
Description	Major urinary proteins (MUP) are the major component of the urinary protein fraction in house mice (Mus spp.) and rats (Rattus spp.). The structure, polymorphism and functions of these lipocalins have been well described in the western European house mouse (Mus musculus domesticus), clarifying their role in semiochemical communication. The complexity of these roles in the mouse raises the question of similar functions in other rodents, including the Norway rat, Rattus norvegicus. Norway rats express MUPs in urine but information about specific MUP isoform sequences and functions is limited. In this study, we present a detailed molecular characterization of the MUP proteoforms expressed in the urine of two laboratory strains, Wistar Han and Brown Norway, and wild caught animals, using a combination of manual gene annotation, intact protein mass spectrometry and bottom-up mass spectrometry-based proteomic approaches. Detailed sequencing of the urinary MUP isoforms reveals a less complex pattern of primary sequence polymorphism in the rat than the mouse. However, rat MUPs exhibit added complexity in the form of post-translational modifications, including the phosphorylation of Ser4 in some isoforms, and exoproteolytic trimming of specific isoforms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:44.670.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Catarina Franco
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-23 07:27:24	ID requested
1	2019-08-01 04:27:59	announced
2	2019-08-03 00:30:37	announced	Updated project metadata.
⏵ 3	2024-10-22 04:55:52	announced	2024-10-22: Updated project metadata.

G, ó, mez-Baena G, Armstrong SD, Halstead JO, Prescott M, Roberts SA, McLean L, Mudge JM, Hurst JL, Beynon RJ, Molecular complexity of the major urinary protein system of the Norway rat, Rattus norvegicus. Sci Rep, 9(1):10757(2019) [pubmed]

10.1038/s41598-019-46950-x;

submitter keyword: 2u-globulins,Rattus norvegicus, phosphorylation, MUPs

Rob J Beynon
contact affiliation	G44, Biosciences Building| Institute of Integrative Biology University of Liverpool Crown Street | Liverpool | L69 7ZB United Kingdom
contact email	r.beynon@liverpool.ac.uk
lab head
Catarina Franco
contact affiliation	Centre for Proteome Research, University of Liverpool
contact email	catarina.franco@liverpool.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013986

PRIDE project URI

• PRIDE
  1. PXD013986
     1. Label: PRIDE project
     2. Name: Molecular complexity of the major urinary protein system of the Norway rat, Rattus norvegicus C4PR_LIV