PXD013967 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Histone octamer structure is altered early in ISW2 ATP-dependent remodeling of nucleosomes |
Description | Histone octamers are thought to be a rigid part of nucleosomes that shape chromatin and block cellular machinery from accessing DNA. ATP-dependent chromatin remodelers like ISW2 mobilize nucleosomes to provide DNA access. We find evidence for histone octamer distortion preceding DNA being moved into nucleosomes and processive movement of the ATPase motor of ISW2 on nucleosomal DNA. DNA entering nucleosome is uncoupled from the ATPase activity of ISW2 and alterations of the histone octamer structure mediated by ISW2 by deletion of the SANT domain from the C-terminus of the Isw2 catalytic subunit. We also find that restricting histone movement by chemical crosslinking traps remodeling intermediates resembling those seen by loss of the SANT domain, further supporting the importance of changes in histone octamer structure early in ISW2 remodeling. Transient octamer distortions are stabilized by H3-H4 tetramer disulfide crosslinking, thereby linking intrinsic histone octamer flexibility to chromatin remodeling. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-27 |
AnnouncementXML | Submission_2020-05-27_01:25:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jie Luo |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-22 03:13:38 | ID requested | |
⏵ 1 | 2020-05-27 01:25:50 | announced | |
Publication List
Hada A, Hota SK, Luo J, Lin YC, Kale S, Shaytan AK, Bhardwaj SK, Persinger J, Ranish J, Panchenko AR, Bartholomew B, Histone Octamer Structure Is Altered Early in ISW2 ATP-Dependent Nucleosome Remodeling. Cell Rep, 28(1):282-294.e6(2019) [pubmed] |
Keyword List
submitter keyword: ISWI, nucleosome dynamics, nucleosome structure, SANT domain, chromatin remodeling, nucleosome movement, histones, gene regulation, transcription |
Contact List
Jeff Ranish |
contact affiliation | Institute For Systems Biology |
contact email | jranish@systemsbiology.org |
lab head | |
Jie Luo |
contact affiliation | Institute for Systems Biology |
contact email | jluo@systemsbiology.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013967 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013967
- Label: PRIDE project
- Name: Histone octamer structure is altered early in ISW2 ATP-dependent remodeling of nucleosomes