PXD013961 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome of L-carnitine-grown Eubacterium limosum ATCC 8486 |
Description | Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of L-carnitine-grown Eubacterium limosum was obtained in order to identify enzymes required for growth on L-carnitine, in particular to identify components that are unique to growth on L-carnitine in comparison to other substrates for acetogenesis, such as lactic acid. L-carnitine and derviatives are converted to trimethylamine (TMA) by certain members of the gut microbiome, metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of carnitine is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Carnitine demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherorsclerosis caused by metabolism of TMA in the body. The carnitine proteome led to the description of a carnitine:tetrahydrofolate methyltransferase system. The key carnitine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-19 |
AnnouncementXML | Submission_2020-06-18_22:10:12.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD013961 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Joseph Krzycki |
SpeciesList | scientific name: Eubacterium limosum; NCBI TaxID: 1736; |
ModificationList | Deamidated; Oxidation; Carbamidomethyl |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-22 01:49:04 | ID requested | |
⏵ 1 | 2020-06-18 22:10:13 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Acetogenic bacteria, human gut isolate, carnitine demethylation |
Contact List
Joseph Adrian Krzycki |
contact affiliation | Department of Microbiology The Ohio State University Columbus OH 43210 |
contact email | Krzycki.1@osu.edu |
lab head | |
Joseph Krzycki |
contact affiliation | The Ohio State University |
contact email | Krzycki.1@osu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013961
- Label: PRIDE project
- Name: Proteome of L-carnitine-grown Eubacterium limosum ATCC 8486