PXD013893-1

PXD013893 is an original dataset announced via ProteomeXchange.

Title	Sequential phosphoproteomics and N-glycoproteomics of plasma-derived extracellular vesicles
Description	Extracellular vesicles (EVs) are increasingly recognized as important cargos for intercellular communication and promising sources for less invasive biomarker discovery. As the state of protein post-translational modifications (PTMs) such as phosphorylation and glycosylation can be a key determinant of cellular physiology such as early stage cancer, comprehensive characterization of protein PTMs in EVs are highly valuable, but significant challenges remain. Recently we demonstrated the feasibility to isolate and identify phosphoproteins and glycoproteins in EVs from human plasma as potential markers to differentiate disease from healthy states. We identified close to 10,000 unique phosphopeptides and 1,500 unique glycopeptides in EVs isolated from small volumes of plasma samples. Using label-free quantitative phospho- and glyco-proteomics, we identified a number of phosphoproteins and glycoproteins in plasma EVs that are significantly higher in patients diagnosed with breast cancer. Here we present an updated workflow to sequentially isolate phosphopeptides and N-glycopeptides, enabling multiple PTM analyses with the same clinical samples and presenting intriguing functional molecules for further validation.
HostingRepository	PRIDE
AnnounceDate	2019-09-25
AnnouncementXML	Submission_2019-09-25_06:45:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hillary Andaluz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; complex glycosylation
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-17 03:19:04	ID requested
⏵ 1	2019-09-25 06:45:30	announced
2	2024-10-22 04:57:21	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: post-translational modification, PTM, glycosylation, phosphorylation, phosphoproteomics, glycoproteomics, extracellular vesicles, exosomes, microvesicle, EV, mass spectrometry, proteomics, liquid chromatography, MS, LC-MS/MS

W. Andy Tao
contact affiliation	Purdue University
contact email	taow@purdue.edu
lab head
Hillary Andaluz
contact affiliation	Purdue University
contact email	handaluz@purdue.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013893

PRIDE project URI

• PRIDE
  1. PXD013893
     1. Label: PRIDE project
     2. Name: Sequential phosphoproteomics and N-glycoproteomics of plasma-derived extracellular vesicles