PXD013823-1

PXD013823 is an original dataset announced via ProteomeXchange.

Title	Intact Transition Epitope Mapping-Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE)
Description	ITEM-THREE is a novel mass spectrometry-based epitope mapping method. To perform ITEM-THREE, antibody-epitope peptide immune-complexes are first generated in electrospray-compatible solutions in which the antibodies maintain their in-solution functionality, by adding an antibody of interest to a mixture of peptides/ digest of antigen from which at least one of the peptides holds the antibody's epitope. This mixture is directly nano-electrosprayed without purification. Identification of the epitope peptide is executed within a mass spectrometer which provides an ion mobility cell sandwiched in-between two collision cells and where this ion manipulation setup is flanked by a quadrupole mass analyzer on one side and a time-of-flight mass analyzer on the other side. In a step-wise fashion, immune-complex ions are separated from unbound peptide ions and dissociated to release epitope peptide ions. Immune complex-released peptide ions are separated from antibody ions and fragmented by collision induced dissociation. Epitope containing peptide fragment ions are recorded and mass lists are submitted to unsupervised data base search thereby retrieving both, the amino acid sequence of the epitope peptide and the originating antigen.
HostingRepository	PRIDE
AnnounceDate	2019-06-03
AnnouncementXML	Submission_2019-06-03_03:54:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-13 06:46:26	ID requested
⏵ 1	2019-06-03 03:54:51	announced

Danquah BD, R, ö, wer C, Opuni KM, El-Kased R, Frommholz D, Illges H, Koy C, Glocker MO, Intact Transition Epitope Mapping - Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE). Mol Cell Proteomics, 18(8):1543-1555(2019) [pubmed]

curator keyword: Technical

submitter keyword: Human, ITEM-THREE, NanoESI, recombinant proteins, collision induced dissociation, ion mobility separation, epitope antibody complexes, molecular recognition

Prof. Dr. Michael O. Glocker
contact affiliation	Proteome Center Rostock Department for Proteome Research Institute of Immunology Medical Faculty and Natural Science Faculty University of Rostock Schillingallee 69 18057 Rostock Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013823

PRIDE project URI

• PRIDE
  1. PXD013823
     1. Label: PRIDE project
     2. Name: Intact Transition Epitope Mapping-Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE)