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PXD013809 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamic rewiring of the human interactome by interferon signalling
DescriptionThe type I interferon (IFN) response protects cells against viral pathogens by inducing the transcription of hundreds of IFN-stimulated genes (ISGs). Transcriptomic and biochemical approaches have established comprehensive catalogues of ISGs across species and cell types, but their antiviral mechanisms remain incompletely characterized. Here, we apply a combination of quantitative proteomic approaches to map IFN-induced rearrangements in the human protein-protein interaction network. Specifically, we analyzed the IFN-induced changes to the HeLa cell proteome with shotgun mass spectrometry. As well, we assessed the IFN-induced interactome with protein correlation profiling coupled to size exclusion chromatography and SILAC. Finally, we utilized label-free quantitative mass spectrometry for affinity purification mass spectrometry of candidate proteins and to analyze the ribosomal protein composition.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJenny Moon
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive; Bruker Daltonics instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-13 05:55:03ID requested
12020-05-15 02:03:14announced
22020-08-11 03:11:50announced2020-08-11: Updated publication reference for PubMed record(s): 32539747.
Publication List
Kerr CH, Skinnider MA, Andrews DDT, Madero AM, Chan QWT, Stacey RG, Stoynov N, Jan E, Foster LJ, Dynamic rewiring of the human interactome by interferon signaling. Genome Biol, 21(1):140(2020) [pubmed]
Keyword List
submitter keyword: interferon, proteomics, interferon stimulated gene, innate immunity, protein correlation profiling, interactome, protein complexes
Contact List
Leonard J. Foster
contact affiliationDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver BC, Canada
contact emailfoster@msl.ubc.ca
lab head
Jenny Moon
contact affiliationUniversity of British Columbia
contact emailkyungmee@mail.ubc.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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