PXD013809-2

PXD013809 is an original dataset announced via ProteomeXchange.

Title	Dynamic rewiring of the human interactome by interferon signalling
Description	The type I interferon (IFN) response protects cells against viral pathogens by inducing the transcription of hundreds of IFN-stimulated genes (ISGs). Transcriptomic and biochemical approaches have established comprehensive catalogues of ISGs across species and cell types, but their antiviral mechanisms remain incompletely characterized. Here, we apply a combination of quantitative proteomic approaches to map IFN-induced rearrangements in the human protein-protein interaction network. Specifically, we analyzed the IFN-induced changes to the HeLa cell proteome with shotgun mass spectrometry. As well, we assessed the IFN-induced interactome with protein correlation profiling coupled to size exclusion chromatography and SILAC. Finally, we utilized label-free quantitative mass spectrometry for affinity purification mass spectrometry of candidate proteins and to analyze the ribosomal protein composition.
HostingRepository	PRIDE
AnnounceDate	2020-05-15
AnnouncementXML	Submission_2020-08-11_03:11:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Moon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; Bruker Daltonics instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-13 05:55:03	ID requested
1	2020-05-15 02:03:14	announced
⏵ 2	2020-08-11 03:11:50	announced	2020-08-11: Updated publication reference for PubMed record(s): 32539747.
3	2024-10-22 05:03:52	announced	2024-10-22: Updated project metadata.

Kerr CH, Skinnider MA, Andrews DDT, Madero AM, Chan QWT, Stacey RG, Stoynov N, Jan E, Foster LJ, Dynamic rewiring of the human interactome by interferon signaling. Genome Biol, 21(1):140(2020) [pubmed]

submitter keyword: interferon, proteomics, interferon stimulated gene, innate immunity, protein correlation profiling, interactome, protein complexes

Leonard J. Foster
contact affiliation	Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver BC, Canada
contact email	foster@msl.ubc.ca
lab head
Jenny Moon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD013809

PRIDE project URI

• PRIDE
  1. PXD013809
     1. Label: PRIDE project
     2. Name: Dynamic rewiring of the human interactome by interferon signalling