PXD013809 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dynamic rewiring of the human interactome by interferon signalling |
Description | The type I interferon (IFN) response protects cells against viral pathogens by inducing the transcription of hundreds of IFN-stimulated genes (ISGs). Transcriptomic and biochemical approaches have established comprehensive catalogues of ISGs across species and cell types, but their antiviral mechanisms remain incompletely characterized. Here, we apply a combination of quantitative proteomic approaches to map IFN-induced rearrangements in the human protein-protein interaction network. Specifically, we analyzed the IFN-induced changes to the HeLa cell proteome with shotgun mass spectrometry. As well, we assessed the IFN-induced interactome with protein correlation profiling coupled to size exclusion chromatography and SILAC. Finally, we utilized label-free quantitative mass spectrometry for affinity purification mass spectrometry of candidate proteins and to analyze the ribosomal protein composition. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-15 |
AnnouncementXML | Submission_2020-08-11_03:11:49.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jenny Moon |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive; Bruker Daltonics instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-13 05:55:03 | ID requested | |
1 | 2020-05-15 02:03:14 | announced | |
⏵ 2 | 2020-08-11 03:11:50 | announced | 2020-08-11: Updated publication reference for PubMed record(s): 32539747. |
3 | 2024-10-22 05:03:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
Kerr CH, Skinnider MA, Andrews DDT, Madero AM, Chan QWT, Stacey RG, Stoynov N, Jan E, Foster LJ, Dynamic rewiring of the human interactome by interferon signaling. Genome Biol, 21(1):140(2020) [pubmed] |
Keyword List
submitter keyword: interferon, proteomics, interferon stimulated gene, innate immunity, protein correlation profiling, interactome, protein complexes |
Contact List
Leonard J. Foster |
contact affiliation | Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver BC, Canada |
contact email | foster@msl.ubc.ca |
lab head | |
Jenny Moon |
contact affiliation | University of British Columbia |
contact email | kyungmee@mail.ubc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013809
- Label: PRIDE project
- Name: Dynamic rewiring of the human interactome by interferon signalling