PXD013782-2

PXD013782 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of DNA repair in Saccharomyces cerevisisae
Description	The role of Rad53 in response to a DNA lesion is central for the accurate orchestration of the DNA damage response. Rad53 activation relies on its phosphorylation by the Mec1 kinase and its own autophosphorylation in a manner dependent on the adaptor Rad9. While the mechanism behind Rad53 phosphorylation and activation has been well documented, less is known about the processes that counteract its kinase activity during the response to a DNA break. Here, we describe that PP4 dephosphorylates Rad53 during the repair of a DNA lesion, a process that affects the phosphorylation status of multiple factors involved in the DNA damage response. PP4-dependent Rad53 dephosphorylation stimulates DNA end resection in a process that relies mainly on Sgs1/Dna2. Consequently, elimination of PP4 activity affects DNA resection and repair by single-strand annealing, defects that are bypassed by reducing the hyper-phosphorylation state of Rad53 observed in the absence of the phosphatase. These results confirms that Rad53 is one of the principal targets of PP4 during the repair of a DNA lesion and demonstrate that the attenuation of its kinase activity during the initial steps of the repair process is essential to efficiently enhance recombinational repair pathways that depend on long-range resection for its execution.
HostingRepository	PRIDE
AnnounceDate	2019-09-12
AnnouncementXML	Submission_2019-10-25_05:10:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Holger Kramer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; N-acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-09 01:47:42	ID requested
1	2019-09-12 05:29:13	announced
⏵ 2	2019-10-25 05:10:55	announced	2019-10-25: Updated publication reference for PubMed record(s): 31544936.
3	2024-10-22 04:15:26	announced	2024-10-22: Updated project metadata.

Villoria MT, Guti, é, rrez-Escribano P, Alonso-Rodr, í, guez E, Ramos F, Merino E, Campos A, Montoya A, Kramer H, Arag, ó, n L, Clemente-Blanco A, PP4 phosphatase cooperates in recombinational DNA repair by enhancing double-strand break end resection. Nucleic Acids Res, 47(20):10706-10727(2019) [pubmed]

submitter keyword: DNA repair/Double-strand break

Homologous recombination

PP4

resection

phosphoproteomics

Andrés Clemente Blanco
contact affiliation	Cell Cycle and Genome Stability Group. Institute of Functional Biology and Genomics (IBFG). Spanish National Research Council (CSIC). University of Salamanca (USAL). C/ Zacarías González 2. Salamanca 37007. SPAIN
contact email	andresclemente@usal.es
lab head
Holger Kramer
contact affiliation	MRC London Institute of Medical Sciences
contact email	h.kramer@lms.mrc.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013782

PRIDE project URI

• PRIDE
  1. PXD013782
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of DNA repair in Saccharomyces cerevisisae