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PXD013773-1

PXD013773 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAmphora coffeaeformis adhesive trail proteomics
DescriptionThroughout all kingdoms of life, a large number of adhesive biomolecules have evolved to allow organisms to adhere to surfaces underwater. Proteins play an important role in the adhesion of numerous marine invertebrates (e.g. mussels, sea stars, sea urchins) whereas much less is known about the biological adhesives from marine plants, including the diatoms. Diatoms are unicellular microalgae that together with bacteria dominate marine biofilms in sunlit habitats. In this study we present the first proteomics analyses of the diatom adhesive material isolated from the tenacious fouling species Amphora coffeaeformis. We identified 21 proteins, of which 13 are diatom specific. Ten of these proteins share a conserved C-terminal domain, termed GDPH domain, which is widespread yet not ubiquitously present in all diatom classes. Immunofluorescence localization of a GDPH domain bearing protein (Ac629) as well as two other proteins identified in this study (Ac1442, Ac9617) demonstrated that these are components of the adhesive trails that are secreted by cells that glide on surfaces.
HostingRepositoryPRIDE
AnnounceDate2022-02-15
AnnouncementXMLSubmission_2022-02-15_09:23:40.261.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNicole Poulsen
SpeciesList scientific name: Amphora coffeiformis; NCBI TaxID: 265554;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrumentimpact HD; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-08 06:29:29ID requested
12022-02-15 09:23:41announced
Publication List
Lachnit M, Buhmann MT, Klemm J, Kr, รถ, ger N, Poulsen N, Identification of proteins in the adhesive trails of the diatom Amphora coffeaeformis. Philos Trans R Soc Lond B Biol Sci, 374(1784):20190196(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: diatom, adhesive
Contact List
Nils Kroger
contact affiliationB CUBE, Technical University, Dresden, Germany
contact emailnils.kroeger@tu-dresden.de
lab head
Nicole Poulsen
contact affiliationTU Dresden
contact emailnicole.poulsen@tu-dresden.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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