PXD013773-1

PXD013773 is an original dataset announced via ProteomeXchange.

Title	Amphora coffeaeformis adhesive trail proteomics
Description	Throughout all kingdoms of life, a large number of adhesive biomolecules have evolved to allow organisms to adhere to surfaces underwater. Proteins play an important role in the adhesion of numerous marine invertebrates (e.g. mussels, sea stars, sea urchins) whereas much less is known about the biological adhesives from marine plants, including the diatoms. Diatoms are unicellular microalgae that together with bacteria dominate marine biofilms in sunlit habitats. In this study we present the first proteomics analyses of the diatom adhesive material isolated from the tenacious fouling species Amphora coffeaeformis. We identified 21 proteins, of which 13 are diatom specific. Ten of these proteins share a conserved C-terminal domain, termed GDPH domain, which is widespread yet not ubiquitously present in all diatom classes. Immunofluorescence localization of a GDPH domain bearing protein (Ac629) as well as two other proteins identified in this study (Ac1442, Ac9617) demonstrated that these are components of the adhesive trails that are secreted by cells that glide on surfaces.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:23:40.261.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicole Poulsen
SpeciesList	scientific name: Amphora coffeiformis; NCBI TaxID: 265554;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact HD; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-08 06:29:29	ID requested
⏵ 1	2022-02-15 09:23:41	announced

Lachnit M, Buhmann MT, Klemm J, Kr, ö, ger N, Poulsen N, Identification of proteins in the adhesive trails of the diatom Amphora coffeaeformis. Philos Trans R Soc Lond B Biol Sci, 374(1784):20190196(2019) [pubmed]

curator keyword: Biological

submitter keyword: diatom, adhesive

Nils Kroger
contact affiliation	B CUBE, Technical University, Dresden, Germany
contact email	nils.kroeger@tu-dresden.de
lab head
Nicole Poulsen
contact affiliation	TU Dresden
contact email	nicole.poulsen@tu-dresden.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD013773

PRIDE project URI

• PRIDE
  1. PXD013773
     1. Label: PRIDE project
     2. Name: Amphora coffeaeformis adhesive trail proteomics