PXD013773 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Amphora coffeaeformis adhesive trail proteomics |
Description | Throughout all kingdoms of life, a large number of adhesive biomolecules have evolved to allow organisms to adhere to surfaces underwater. Proteins play an important role in the adhesion of numerous marine invertebrates (e.g. mussels, sea stars, sea urchins) whereas much less is known about the biological adhesives from marine plants, including the diatoms. Diatoms are unicellular microalgae that together with bacteria dominate marine biofilms in sunlit habitats. In this study we present the first proteomics analyses of the diatom adhesive material isolated from the tenacious fouling species Amphora coffeaeformis. We identified 21 proteins, of which 13 are diatom specific. Ten of these proteins share a conserved C-terminal domain, termed GDPH domain, which is widespread yet not ubiquitously present in all diatom classes. Immunofluorescence localization of a GDPH domain bearing protein (Ac629) as well as two other proteins identified in this study (Ac1442, Ac9617) demonstrated that these are components of the adhesive trails that are secreted by cells that glide on surfaces. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-15 |
AnnouncementXML | Submission_2022-02-15_09:23:40.261.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nicole Poulsen |
SpeciesList | scientific name: Amphora coffeiformis; NCBI TaxID: 265554; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | impact HD; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-08 06:29:29 | ID requested | |
⏵ 1 | 2022-02-15 09:23:41 | announced | |
Publication List
Lachnit M, Buhmann MT, Klemm J, Kr, รถ, ger N, Poulsen N, Identification of proteins in the adhesive trails of the diatom Amphora coffeaeformis. Philos Trans R Soc Lond B Biol Sci, 374(1784):20190196(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: diatom, adhesive |
Contact List
Nils Kroger |
contact affiliation | B CUBE, Technical University, Dresden, Germany |
contact email | nils.kroeger@tu-dresden.de |
lab head | |
Nicole Poulsen |
contact affiliation | TU Dresden |
contact email | nicole.poulsen@tu-dresden.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD013773 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013773
- Label: PRIDE project
- Name: Amphora coffeaeformis adhesive trail proteomics