PXD013762-1

PXD013762 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	HUVEC mCherry or GPNMB-mChery over expressed, mCherry-IP
Description	HUVEC were infected retroviral vectors expressing mCherry-GPNMB or mCherry. The cells were lysed with Pierce IP lysis buffer. The lysates after immunoprecipitation using anti-mCherry antibody (ab213511, Abcam) was transferred into a new microcentrifuge tube for “tube gel digestion”, a modified in-gel digestion procedure, to generate dithiothreitol-reduced iodoacetamide-alkylated tryptic peptides for nano-flow liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).
HostingRepository	jPOST
AnnounceDate	2022-05-08
AnnouncementXML	Submission_2022-05-07_08:00:04.368.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Masayoshi Suda
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; unknown modification
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-08 01:18:20	ID requested
⏵ 1	2022-05-07 08:00:04	announced
2	2022-09-18 02:20:34	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: GPNMB, HUVEC

submitter keyword: GPNMB, HUVEC

Contact List

Tohru Minamino
lab head
Masayoshi Suda
contact affiliation	Niigata University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000594

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000594