PXD013731 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Functional characterization of the p97/valosin-containing protein (VCP) interacting network in Leishmania Functional characterization of the p97/valosin-containing protein (VCP) interacting network in Leishmania |
Description | Valosin‐containing protein (VCP)/p97/Cdc48 is an essential AAA+ ATPase associated with many ubiquitin-dependent cellular pathways that plays a central role in protein quality control in all eukaryotes. Its functional diversity relies on its ability to cooperate with a large number of protein cofactors that provide pathway selectivity and fine-tune substrate processing. Here, we identified the main partners of the recently characterized Leishmania infantum VCP homolog (LiVCP) and we provide the first insights into the biology of the LiVCP network in Trypanosomatidae. Among the LiVCP core partners detected by immunoprecipitation (IP) and LC-MS/MS mass spectra acquisition, we determined p47, FAF1, UFD1 and PUB1 as the major cofactors of the Leishmania VCP. Furthermore, we provide in silico analyses demonstrating the conserved regions of these cofactors that potentially interact with LiVCP. We employed ‘‘network proteomics’’ using IP and LC-MS/MS studies for each cofactor to confirm their close partnership with LiVCP and to identify the cofactor-specific interacting partners as well as the partners that are shared among multiple cofactors and LiVCP complexes, such as the important heterotrimer VCP-NPL4-UFD1 complex in Leishmania. Our results suggest a glycosome/peroxisome association of LiFAF1. Gene Ontology analysis of each proteome coupled with digitonin fractionation and immunofluorescence studies indicated nuclear association for Lip47, cytoplasmic and vacuolar association for LiUFD1, glycosome association for LiFAF1, and endoplasmic reticulum interaction for LiPUB1 protein. All together, these data provide the first VCP protein network in Trypanosomatidae. Further functional and structural analysis of the essential LiVCP quality control protein network may lead to the development of new anti-parasitic drugs. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-11 |
AnnouncementXML | Submission_2020-08-11_02:35:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bruno Aguiar |
SpeciesList | scientific name: Leishmania infantum JPCM5; NCBI TaxID: 435258; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-07 02:54:41 | ID requested | |
⏵ 1 | 2020-08-11 02:35:22 | announced | |
Publication List
Aguiar BG, Dumas C, Maaroufi H, Padmanabhan PK, Papadopoulou B, ATPase valosin-containing protein (VCP)/p97/Cdc48 interaction network in Leishmania. Sci Rep, 10(1):13135(2020) [pubmed] |
Keyword List
submitter keyword: Leishmania |
VCP/p97 |
VCP cofactors |
co-immunoprecipitation |
LC-MS/MS |
Network proteomics |
Contact List
Barbara Papadopoulou |
contact affiliation | CHU de Quebec Research Center-Laval University 2705 Laurier Blvd., Quebec (QC), Canada G1V 4G2 Phone: (418) 525-4444, ext. 47608; Fax: (418) 654-2715 |
contact email | barbara.papadopoulou@crchudequebec.ulaval.ca |
lab head | |
Bruno Aguiar |
contact affiliation | CHUL-QUÉBEC |
contact email | guedesaguiar@ufpi.edu.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013731
- Label: PRIDE project
- Name: Functional characterization of the p97/valosin-containing protein (VCP) interacting network in Leishmania Functional characterization of the p97/valosin-containing protein (VCP) interacting network in Leishmania