PXD013727-1

PXD013727 is an original dataset announced via ProteomeXchange.

Title	¬¬Identifying the cellular interactome of Epstein-Barr virus lytic regulator Zta reveals cellular targets contributing to viral replication.
Description	The human gamma herpesvirus Epstein-Barr virus, infects most adults and is an important contributor to the development of many types of cancer. Essential contributions of viral genes to replication are known but the potential contributions of cell genes to viral replication are less well delineated. A key player is the viral protein Zta (BZLF1, ZEBRA, Z). This sequence-specific DNA-binding protein can disrupt viral latency by driving the transcription of target genes and by interacting with the EBV lytic origin of replication. Here we used an unbiased approach to identify the Zta-interactome in cells derived from a Burkitt’s lymphoma. Isolating Zta and associated proteins from Burkitt’s lymphoma cells undergoing EBV replication, followed by Tandem Mass Tag (TMT) mass spectrometry resulted in the identification of forty-four viral and cellular proteins in the Zta interactome. Of these two were known targets of Zta. The association of Zta with Hsc70 and the contribution that Hsc70 plays to EBV replication mirrors a contribution from HSP70 family members to the replication of other herpesviruses. Conversely, the association of Zta with NFATc2 has no known parallels for other herpesviruses. Zta attenuates the activity of an NFAT-dependent promoter, which shows a potential for dampening the expression of genes regulated by calcium-dependent signal transduction. Indeed, Zta has the ability to affect a feed-back loop limiting its own expression, which would aid viral replication by preventing the toxic effects of Zta overexpression.
HostingRepository	PRIDE
AnnounceDate	2019-06-06
AnnouncementXML	Submission_2019-06-06_05:49:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alison Sinclair
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human herpesvirus 4 type 1; NCBI TaxID: 36352;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-07 02:42:52	ID requested
⏵ 1	2019-06-06 05:50:32	announced
2	2024-10-22 04:56:32	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: EBV

BZLF1

lytic replication;NFAT;HSC70

Alison Sinclair
contact affiliation	School of Life Sciences University of Sussex Brighton BN1 9QG UK
contact email	a.j.sinclair@sussex.ac.uk
lab head
Alison Sinclair
contact affiliation	University of Sussex
contact email	a.j.sinclair@sussex.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013727
     1. Label: PRIDE project
     2. Name: ¬¬Identifying the cellular interactome of Epstein-Barr virus lytic regulator Zta reveals cellular targets contributing to viral replication.