PXD013701-2

PXD013701 is an original dataset announced via ProteomeXchange.

Title	Energetics- and transport-related protein abundance responds differentially to salt in Oryza australiensis roots
Description	Salinity is a major constraint on agricultural productivity worldwide. Despite the serious impacts of salinity on rice yields, particularly in Asia, mechanisms of salt tolerance in wild rice species are yet to be explored. Here we extracted and quantified root microsomal proteins of Oryza australiensis accessions contrasting in salt tolerance. Whole root systems of two-week-old seedlings were treated with 80 mM NaCl for 30 days or left untreated. Proteins were quantified by tandem mass tags (TMT) and triple-stage MS. We found >200 differentially expressed proteins (DEPs) between the salt-treated and control in the two accessions (p-value <0.05). Gene Ontology (GO) analysis showed that ‘metabolic process’, ‘transport’ and ‘transmembrane transporter’ activities were highly responsive categories following salt treatment of the O. australiensis seedlings. In particular, ATPases and SNARE proteins were up-regulated in the salt-tolerant accession and appeared to have a major role in response to salinity. ATPases are the central link between energy generation and transport, while SNARE proteins facilitate vesicle fusion and interact with voltage-gated potassium channels to regulate K+ influx. We successfully validated the putative function of two strongly upregulated proteins, a monosaccharide transporter and a VAMP-like protein, by measuring the growth under salinity of yeast mutants in which homologous genes were deleted. Our results demonstrate the potential use of wild species as a source of new mechanisms of salt tolerance for the breeding of elite cultivars of rice.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:56:26.688.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yoav Yichie
SpeciesList	scientific name: Oryza australiensis; NCBI TaxID: 4532;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-03 03:44:03	ID requested
1	2019-09-11 10:19:43	announced
⏵ 2	2024-10-22 04:56:27	announced	2024-10-22: Updated project metadata.

Yichie Y, Hasan MT, Tobias PA, Pascovici D, Goold HD, Van Sluyter SC, Roberts TH, Atwell BJ, Salt-Treated Roots of Oryza australiensis Seedlings are Enriched with Proteins Involved in Energetics and Transport. Proteomics, 19(19):e1900175(2019) [pubmed]

10.1002/pmic.201900175;

10.1186/s12284-018-0257-7;

Yichie Y, Brien C, Berger B, Roberts TH, Atwell BJ, Salinity tolerance in Australian wild Oryza species varies widely and matches that observed in O. sativa. Rice (N Y), 11(1):66(2018) [pubmed]

submitter keyword: Oryza sativa, rice, ATPase, proteomics, Oryza australiensis, transporters, energy metabolism,Stress response, tolerance, tandem mass tags

Thomas H Roberts
contact affiliation	Sydney Institute of Agriculture, University of Sydney, Sydney, Australia
contact email	thomas.roberts@sydney.edu.au
lab head
Yoav Yichie
contact affiliation	University of Sydney
contact email	yoav.yichie@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD013701

PRIDE project URI

• PRIDE
  1. PXD013701
     1. Label: PRIDE project
     2. Name: Energetics- and transport-related protein abundance responds differentially to salt in Oryza australiensis roots