PXD013697-1
PXD013697 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Oxygen-dependent interaction between FBXL5 and CIA targeting complex regulates iron homeostasis |
| Description | The iron sensing protein FBXL5 is the substrate adaptor for a SKP1-CUL1-RBX1 E3 ubiquitin ligase complex that regulates the degradation of iron regulatory proteins (IRPs). Here we describe a mechanism of FBXL5 regulation involving its interaction with the cytosolic Fe-S cluster assembly (CIA) targeting complex comprised of MMS19, FAM96B, and CIAO1. We demonstrate that the CIA targeting complex promotes the ability of FBXL5 to degrade IRPs. In addition, the FBXL5-CIA targeting complex interaction is regulated by oxygen tension displaying a robust association in 21% O2 that is severely diminished in 1% O2 and contributes to O2-dependent regulation of IRP degradation. Together, these data identify a novel oxygen-dependent signaling axis that links IRP-dependent iron homeostasis with the Fe-S cluster assembly machinery. |
| HostingRepository | MassIVE |
| AnnounceDate | 2019-05-02 |
| AnnouncementXML | Submission_2019-05-02_18:05:14.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | William Barshop |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl |
| Instrument | instrument model: Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-05-02 17:34:20 | ID requested | |
| ⏵ 1 | 2019-05-02 18:05:15 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: FBXL5, IRP, MMS19, FAM96B, CIAO1, CIA Targeting Complex, Iron Homeostasis, Hypoxia, Poly-ubiquitination, Iron Sensor, CIA Targeting Complex Binding Domain |
Contact List
| James A. Wohlschlegel | |
|---|---|
| contact affiliation | University of California, Los Angeles |
| contact email | jwohl@ucla.edu |
| lab head | |
| William Barshop | |
| contact affiliation | UCLA |
| contact email | wbarshop@ucla.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083739 |
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