PXD013662-1

PXD013662 is an original dataset announced via ProteomeXchange.

Title	Identification of mouse embryonic fibroblast secreted protein factors that are responsible for reprogramming human pluripotent stem cell metabolism
Description	Previously we found that human pluripotent stem cells (hPSCs) utilize glucose differently depending on the presence of the feeder cells, which are mouse embryonic fibroblasts, or MEFs. More specifically, feeder-free cultured hPSCs are more reliant on glycolysis for proliferation. Therefore, we hypothesized that secreted factors by MEFs might be responsible for reprogramming the metabolism of hPSCs. To test this hypothesis, we separated the components in the MEF-conditioned medium by using size-based fractionation columns, and tested whether each fraction alters the reliance of feeder-free hPSCs on glucose. We concluded that it was the protein fraction of the MEF-conditioned medium potentially responsible for reprogramming glycolytic metabolism in hPSCs. To further understand which specific protein(s) could alter the metabolism of hPSCs, we here conduct mass spectrometry based proteomics experiment.
HostingRepository	PRIDE
AnnounceDate	2022-04-01
AnnouncementXML	Submission_2022-04-01_09:37:28.225.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wen Gu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-30 02:36:06	ID requested
⏵ 1	2022-04-01 09:37:29	announced
2	2024-10-22 05:35:40	announced	2024-10-22: Updated project metadata.

Teo GC, Polasky DA, Yu F, Nesvizhskii AI, Fast Deisotoping Algorithm and Its Implementation in the MSFragger Search Engine. J Proteome Res, 20(1):498-505(2021) [pubmed]

submitter keyword: mouse embryonic fibroblast, human pluripotent stem cell metabolism

Wen Gu
contact affiliation	UT Southwestern Medical Center
contact email	wen.gu@utsouthwestern.edu
lab head
Wen Gu
contact affiliation	UT Southwestern Medical Center
contact email	wen.gu@utsouthwestern.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD013662

PRIDE project URI

• PRIDE
  1. PXD013662
     1. Label: PRIDE project
     2. Name: Identification of mouse embryonic fibroblast secreted protein factors that are responsible for reprogramming human pluripotent stem cell metabolism