PXD013642 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of Rhizobium favelukesii LPU83 in response to acid stress. |
Description | Acid soils constitute a severe problem for leguminous crops mainly through a disturbance in rhizobia-legume interactions. Rhizobium favelukesii—an acid-tolerant rhizobium able to nodulate alfalfa—is highly competitive for nodule occupation under acid conditions, but inefficient in biologic nitrogen fixation. In this work, we obtained a general description of the acid-stress response of R. favelukesii LPU83 by means of proteomics by comparing the total proteome profiles in the presence or absence of acid stress by nanoflow ultrahigh-performance liquid chromatography coupled to mass spectrometry. Thus, a total of 336 proteins were identified with a significant differential expression, 136 of which species were significantly overexpressed and 200 underexpressed in acidity. An in-silico functional characterization with those respective proteins revealed a complex and pleiotropic response by these rhizobia involving components of oxidative phosphorylation, glutamate metabolism, and peptidoglycan biosynthesis, among other pathways. Furthermore, a lower permeability was evidenced in the acid-stressed cells along with several overexpressed proteins related to γ-aminobutyric-acid metabolism, such as the gene product of livK, which was mutated. This mutant exhibited an acid-sensitive phenotype in agreement with the proteomics results. We conclude that both γ-aminobutyric-acid metabolism and a modified cellular envelope could be relevant to acid tolerance in R. favelukesii. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-08 |
AnnouncementXML | Submission_2022-04-08_13:44:32.795.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Juliet Nilsson |
SpeciesList | scientific name: Rhizobium favelukesii; NCBI TaxID: 348824; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-26 01:40:10 | ID requested | |
⏵ 1 | 2022-04-08 13:44:33 | announced | |
2 | 2024-10-22 05:35:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
Nilsson JF, Castellani LG, Draghi WO, P, é, rez-Gim, é, nez J, Torres Tejerizo GA, Pistorio M, LPU83 in Response to Acid Stress. J Proteome Res, 18(10):3615-3629(2019) [pubmed] |
Keyword List
submitter keyword: Acid-stress rhizobia proteomics |
Contact List
Mariano Pistorio |
contact affiliation | Instituto de Biotecnología y Biología Molecular. Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata. CONICET. |
contact email | pistorio@biol.unlp.edu.ar |
lab head | |
Juliet Nilsson |
contact affiliation | Instituto de Biotecnologia y Biologia Molecular (IBBM) -CCT La Plata CONICET- Fac. de Cs. Exactas, UNLP. |
contact email | julietnilsson@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013642
- Label: PRIDE project
- Name: Proteomic analysis of Rhizobium favelukesii LPU83 in response to acid stress.