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PXD013642-1

PXD013642 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of Rhizobium favelukesii LPU83 in response to acid stress.
DescriptionAcid soils constitute a severe problem for leguminous crops mainly through a disturbance in rhizobia-legume interactions. Rhizobium favelukesii—an acid-tolerant rhizobium able to nodulate alfalfa—is highly competitive for nodule occupation under acid conditions, but inefficient in biologic nitrogen fixation. In this work, we obtained a general description of the acid-stress response of R. favelukesii LPU83 by means of proteomics by comparing the total proteome profiles in the presence or absence of acid stress by nanoflow ultrahigh-performance liquid chromatography coupled to mass spectrometry. Thus, a total of 336 proteins were identified with a significant differential expression, 136 of which species were significantly overexpressed and 200 underexpressed in acidity. An in-silico functional characterization with those respective proteins revealed a complex and pleiotropic response by these rhizobia involving components of oxidative phosphorylation, glutamate metabolism, and peptidoglycan biosynthesis, among other pathways. Furthermore, a lower permeability was evidenced in the acid-stressed cells along with several overexpressed proteins related to γ-aminobutyric-acid metabolism, such as the gene product of livK, which was mutated. This mutant exhibited an acid-sensitive phenotype in agreement with the proteomics results. We conclude that both γ-aminobutyric-acid metabolism and a modified cellular envelope could be relevant to acid tolerance in R. favelukesii.
HostingRepositoryPRIDE
AnnounceDate2022-04-08
AnnouncementXMLSubmission_2022-04-08_13:44:32.795.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJuliet Nilsson
SpeciesList scientific name: Rhizobium favelukesii; NCBI TaxID: 348824;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-26 01:40:10ID requested
12022-04-08 13:44:33announced
22024-10-22 05:35:55announced2024-10-22: Updated project metadata.
Publication List
Nilsson JF, Castellani LG, Draghi WO, P, é, rez-Gim, é, nez J, Torres Tejerizo GA, Pistorio M, LPU83 in Response to Acid Stress. J Proteome Res, 18(10):3615-3629(2019) [pubmed]
Keyword List
submitter keyword: Acid-stress rhizobia proteomics
Contact List
Mariano Pistorio
contact affiliationInstituto de Biotecnología y Biología Molecular. Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata. CONICET.
contact emailpistorio@biol.unlp.edu.ar
lab head
Juliet Nilsson
contact affiliationInstituto de Biotecnologia y Biologia Molecular (IBBM) -CCT La Plata CONICET- Fac. de Cs. Exactas, UNLP.
contact emailjulietnilsson@gmail.com
dataset submitter
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Dataset FTP location
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