PXD013626-2

PXD013626 is an original dataset announced via ProteomeXchange.

Title	RNA-Binding Motif protein 6 is a candidate serum biomarker for pancreatic cancer
Description	Early diagnosis is crucial to improve outcomes for pancreatic cancer (PC) patients, however the lack of specific and validated biomarkers for the disease remains challenging. In this study, we analyzed the serum proteome of 12 PC patients and 76 healthy controls (HC), using magnetic bead-based weak cation exchange and matrix-assisted laser desorption ionization-time of flight mass spectrometry. Next, we established supervised neural network (SNN) algorithm model by ClinProTools and identified the candidate biomarker using liquid chromatography-electrospray ionization-tandem mass spectrometry. Finally, candidate biomarker was validated in tissue samples. The SNN algorithm model discriminated PC from HC with 92.97% sensitivity and 94.55% specificity. We identified 115 differentially expressed peptides, nine of which were significantly different (P<0.005). We identified three upregulated peaks (peaks 2, 8 and 9) and six downregulated peaks (peaks 1, 3, 4, 5, 6 and 7) in samples from PC patients, compared with HC samples. Interestingly, one peptide demonstrated the most significant changes between PC and HC samples, which we identified as regions of RNA-binding motif protein 6 (RBM6). In subsequent tissue analysis, we verified RBM6 expression was significantly higher in PC tissues than paracancerous tissue. Our results indicate that RBM6 might serve as a candidate diagnostic biomarker for PC.
HostingRepository	iProX
AnnounceDate	2019-04-25
AnnouncementXML	Submission_2019-12-10_20:18:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Baojun Duan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-24 23:26:07	ID requested
1	2019-04-24 23:29:43	announced
⏵ 2	2019-12-10 20:18:35	announced	2019-12-11: Update publication information.

Duan B, Hu X, Fan M, Xiong X, Han L, Wang Z, Tong D, Liu L, Wang X, Li W, Yang J, Huang C, RNA-Binding Motif Protein 6 is a Candidate Serum Biomarker for Pancreatic Cancer. Proteomics Clin Appl, 13(5):e1900048(2019) [pubmed]

submitter keyword: Pancreatic cancer, Proteomics, Serum biomarker, RBM6

Chen Huang
contact affiliation	Key Laboratory of Environment and Disease-Related Gene, Ministry of Education/Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi’an Jiaotong University, Health Science Center
contact email	hchen@mail.xjtu.edu.cn
lab head
Baojun Duan
contact affiliation	Shaanxi provincail people's hospital
contact email	duanbaojun.cn@163.com
dataset submitter

iProX dataset URI