PXD013602-2

PXD013602 is an original dataset announced via ProteomeXchange.

Title	Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes
Description	Extracellular vesicles are small (50-200 nm diameter) membrane-bound structures released by cells from all domains of life. Vesicles have been implicated in numerous microbial processes, including gene transfer, signaling, pathogenesis, and defense. While vesicles are abundant in the oceans, little is known about their contents or whether they interact with cells in this dilute environment. Here we show that extracellular vesicles contain a remarkable diversity of organic carbon compounds and likely mediate a complex network of export, transport and exchange within marine microbial communities. We report that vesicles from selected strains of Prochlorococcus, the most abundant cyanobacterium in the sea, and other bacteria can interact with cells of various marine microbes, including the numerically abundant heterotroph Pelagibacter. Through a combination of lipidomic, metabolomic and proteomic analyses, we show that two strains of Prochlorococcus export an enormous array of organic and inorganic compounds – including active, biogeochemically relevant enzymes – within vesicles. Vesicles from both strains contained some materials in common as well as numerous strain-specific differences, reflecting an even greater degree of functional complexity within natural vesicle populations. These data suggest additional roles for vesicles in carrying out extracellular biogeochemical reactions, mitigating toxicity from reactive oxygen species, and mediating energy and nutrient transfers in the oceans.
HostingRepository	PRIDE
AnnounceDate	2024-08-08
AnnouncementXML	Submission_2024-08-08_02:33:30.020.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Lundeen
SpeciesList	scientific name: Prochlorococcus marinus str. MIT 9313; NCBI TaxID: 74547; scientific name: Prochlorococcus marinus str. MIT 9312; NCBI TaxID: 74546;
ModificationList	dehydroalanine (Ser); dehydrobutyrine (Thr); iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-23 05:43:50	ID requested
1	2021-11-23 07:37:41	announced
⏵ 2	2024-08-08 02:33:30	announced	2024-08-08: Updated project metadata.

Dataset with its publication pending

submitter keyword: Prochlorococcus, MIT9312, shotgun proteomics, marine cyanobacteria, MIT9313, extracellular vesicles

Anitra Ingalls
contact affiliation	University of Washington
contact email	aingalls@uw.edu
lab head
Rachel Lundeen
contact affiliation	University of Washington
contact email	ralundeen@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD013602

PRIDE project URI

• PRIDE
  1. PXD013602
     1. Label: PRIDE project
     2. Name: Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes