PXD013592-2

PXD013592 is an original dataset announced via ProteomeXchange.

Title	The jasmonate regulome: Reconstructing a hormone signalling pathway from transcription factor activity to phenotypic outputs
Description	We have investigated the genome regulatory network that controls responses to the hormone jasmonate in seedlings. There is much interest currently in understanding the systems-level actions of transcription factors. However, such studies can provide much greater biological insight if set in the context of the signal transduction pathway that initiates gene expression changes and the downstream organismal phenotypes they control. We have generated an integrated framework of the response to jasmonate that spans from signal perception and transduction, through activity of master and secondary-regulatory transcription factors, to gene expression outputs and chromatin remodelling. We make use of genome-wide target maps for hundreds of transcription factors, dynamic histone modification analysis, global proteome and phosphoproteome analyses, massively multiplexed protein-protein interactome data and time series transcriptome analysis. This enables us to predict previously unknown components of the jasmonate regulatory mechanism and validate them through targeted mutant studies. The result is a comprehensive understanding of how a plant hormone remodels cellular function and plant behaviour.
HostingRepository	PRIDE
AnnounceDate	2020-03-19
AnnouncementXML	Submission_2020-06-12_02:43:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013592
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Moresco
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; Phospho; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-23 02:29:58	ID requested
1	2020-03-19 03:26:43	announced
⏵ 2	2020-06-12 02:43:34	announced	2020-06-12: Updated publication reference for PubMed record(s): 32170290.

Zander M, Lewsey MG, Clark NM, Yin L, Bartlett A, Saldierna Guzm, á, n JP, Hann E, Langford AE, Jow B, Wise A, Nery JR, Chen H, Bar-Joseph Z, Walley JW, Solano R, Ecker JR, Integrated multi-omics framework of the plant response to jasmonic acid. Nat Plants, 6(3):290-302(2020) [pubmed]

submitter keyword: Arabidopsis thaliana, Jasmonic acid, MYC2, regulome

Joseph R. Ecker
contact affiliation	Salk Institute of Biological Studies Howard Hughes Medical Institute
contact email	ecker@salk.edu
lab head
James Moresco
contact affiliation	The Salk Institute for Biological Studies
contact email	jmoresco@salk.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD013592

PRIDE project URI

• PRIDE
  1. PXD013592
     1. Label: PRIDE project
     2. Name: The jasmonate regulome: Reconstructing a hormone signalling pathway from transcription factor activity to phenotypic outputs