PXD013585-2

PXD013585 is an original dataset announced via ProteomeXchange.

Title	Musashi interaction with poly(A) binding protein is required for activation of target mRNA translation
Description	The Musashi family of mRNA translational regulators control both physiological and pathological stem cell self-renewal primarily by repressing targets that promote differentiation. In response to differentiation cues, Musashi can switch from a repressor to an activator of target mRNA translation. However, the molecular events that distinguish Musashi-mediated translational activation from repression are not understood. We have previously reported that Musashi function is required for the maturation of Xenopus oocytes, and specifically for translational activation of specific dormant maternal mRNAs. Here, we employed mass spectrometry to identify cellular factors necessary for Musashi-dependent mRNA translational activation. We report a requirement for association of Musashi1 with the embryonic poly(A) binding protein (ePABP) or the canonical somatic cell poly(A) binding protein PABPC1 for activation of Musashi target mRNA translation. Co-immunoprecipitation studies demonstrated an increased Musashi1 interaction with ePABP during oocyte maturation. Attenuation of endogenous ePABP activity severely compromised Musashi function, preventing downstream signaling and blocking oocyte maturation. Recovery of Musashi-dependent mRNA translational activation and maturation of ePABP attenuated oocytes was achieved through ectopic expression of either ePABP or PABPC1. Consistent with the findings in Xenopus oocytes, PABPC1 remained associated with Musashi under conditions of Musashi target mRNA de-repression and translation during mammalian stem cell differentiation. Since association of Musashi1 with poly(A) binding proteins has previously only been implicated in repression of Musashi target mRNAs, our findings reveal distinct context-dependent roles for the interaction of Musashi with poly[A] binding protein family members in response to extracellular cues that control cell fate.
HostingRepository	PRIDE
AnnounceDate	2019-05-31
AnnouncementXML	Submission_2019-06-06_07:31:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013585
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stephanie Byrum
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	Oxidation
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-23 01:14:31	ID requested
1	2019-05-31 03:20:58	announced
⏵ 2	2019-06-06 07:47:21	announced	Updated publication reference for PubMed record(s): 31152063.
3	2023-11-14 09:01:17	announced	2023-11-14: Updated project metadata.

10.6019/PXD013585;

Cragle CE, MacNicol MC, Byrum SD, Hardy LL, Mackintosh SG, Richardson WA, Gray NK, Childs GV, Tackett AJ, MacNicol AM, Musashi interaction with poly(A)-binding protein is required for activation of target mRNA translation. J Biol Chem, 294(28):10969-10986(2019) [pubmed]

submitter keyword: mRNA translation, Musashi, PABP, stem cell, oocyte

Angus M. MacNicol
contact affiliation	Department of Neurobiology and Developmental Science, University of Arkansas for Medical Sciences, Little Rock, AR
contact email	Angus@UAMS.edu
lab head
Stephanie Byrum
contact affiliation	UAMS
contact email	sbyrum@uams.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013585
     1. Label: PRIDE project
     2. Name: Musashi interaction with poly(A) binding protein is required for activation of target mRNA translation