PXD013536-1

PXD013536 is an original dataset announced via ProteomeXchange.

Title	Multifaceted Mass Spectrometric Investigation of Neuropeptide Changes in Atlantic Blue Crab, Callinectes sapidus, in Response to Low pH Stress
Description	The decrease of pH level in the water affects animals living in aquatic habitat, such as crustaceans. The molecular mechanisms enabling these animals to survive this environmental stress remain unknown. To understand the modulatory function of neuropeptides in crustaceans when encountering drops in pH level, we developed and implemented a multifaceted mass spectrometric platform to investigate the global neuropeptide changes in response to water acidification in the Atlantic blue crab, Callinectes sapidus. Neural tissues were collected at different incubation periods to monitor dynamic changes of neuropeptides under different stress conditions occurring in the animal. Neuropeptide families were found to exhibit distinct expression patterns in different tissues and even each isoform had its specific response to the stress. Circulating fluid in the crabs (hemolymph) was also analyzed after 2-hour exposure to acidification condition, and together with results from tissue analysis, enabled the discovery of neuropeptides participating in the stress accommodation process as putative hormones. Two novel peptide sequences were detected in the hemolymph that appeared to be involved in the stress-related regulation in the crabs.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_10:03:40.789.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Liu
SpeciesList	scientific name: Crustacea; NCBI TaxID: 6657;
ModificationList	amidated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-17 03:59:10	ID requested
⏵ 1	2021-09-08 10:03:41	announced
2	2024-10-22 05:26:11	announced	2024-10-22: Updated project metadata.

Liu Y, Buchberger AR, DeLaney K, Li Z, Li L, Multifaceted Mass Spectrometric Investigation of Neuropeptide Changes in Atlantic Blue Crab, Callinectes sapidus, in Response to Low pH Stress. J Proteome Res, 18(7):2759-2770(2019) [pubmed]

submitter keyword: Neuropeptide, crustacean, mass spectrometry

Lingjun Li
contact affiliation	School of Pharmacy and Department of Chemistry University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Yang Liu
contact affiliation	University of Wisconsin, Madison
contact email	liu488@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013536
     1. Label: PRIDE project
     2. Name: Multifaceted Mass Spectrometric Investigation of Neuropeptide Changes in Atlantic Blue Crab, Callinectes sapidus, in Response to Low pH Stress