PXD013518-2

PXD013518 is an original dataset announced via ProteomeXchange.

Title	Transcriptome and proteome profiles of the diazotroph Nitrospirillum amazonense strain CBAmC in response to the sugarcane apoplast fluid
Description	The diazotrophic CBAmC strain of Nitrospirillum amazonense has been reported to promote growth of sugarcane variety RB867515 when inoculated under field conditions in a consortium with four other nitrogen-fixing bacteria. The present work had the objective of accessing the influence of the sugarcane apoplast fluid from variety RB867515 on the global transcriptomic and proteomic profiles of CBAmC strain cultured in vitro. The comparative analysis between the transcriptome and proteome profiles showed strong consistency between the results of the two approaches. Data analysis revealed that expression levels of transcripts and proteins related to cell motility and signal transduction, among other functions, were strongly downregulated in the presence of the apoplast fluid. In contrast, transcripts and proteins were upregulated for functional categories including chaperones, iron homeostasis and multidrug efflux transporters. In conclusion, the results showed that the metabolic state of N. amazonense during cultivation in the presence of the apoplast fluid is directed to functions capable of supporting adaptation to the complex environment of the sugarcane apoplast.
HostingRepository	PRIDE
AnnounceDate	2019-07-12
AnnouncementXML	Submission_2019-08-19_02:43:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schwab
SpeciesList	scientific name: Nitrospirillum amazonense CBAmc; NCBI TaxID: 1441467;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-16 01:40:41	ID requested
1	2019-07-12 03:36:29	announced
⏵ 2	2019-08-19 02:44:00	announced	Updated publication reference for DOI(s): 10.1007/s11104-019-04201-y.
3	2023-11-14 07:01:27	announced	2023-11-14: Updated project metadata.
4	2024-10-22 04:55:23	announced	2024-10-22: Updated project metadata.

10.1007/s11104-019-04201-y;

submitter keyword: Transcripts

proteins

bacteria-plant interaction

Saccharum spp.

Stefan Schwab
contact affiliation	Embrapa Agrobiologia, Seropédica-RJ, Brazil.
contact email	stefan.schwab@embrapa.br
lab head
Stefan Schwab
contact affiliation	Embrapa
contact email	stefan.schwab@embrapa.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013518
     1. Label: PRIDE project
     2. Name: Transcriptome and proteome profiles of the diazotroph Nitrospirillum amazonense strain CBAmC in response to the sugarcane apoplast fluid