PXD013502-1

PXD013502 is an original dataset announced via ProteomeXchange.

Title	In vivo murine brain protein synthesis uncover the molecular physiological process in brain
Description	Identification of proteins that are synthesised de novo in response to specific microenvironmental cues is critical for understanding the molecular mechanisms, which underpin vital physiological processes and pathologies. Here, we report that a brief period of pulsed SILAM (Stable Isotope Labeling of Mammals) diet enables determination of biological functions corresponding to actively translating proteins in the mouse brain. Our results demonstrate that the synapse, dendrite and myelin sheath are highly active cellular compart of protein synthesis, rapidly expressing key mediators of nutrient sensing, lipid metabolism, amyloid precursor proteins processing and stability. Together, these findings confirm that protein metabolic activity varies significantly between brain cellular compartments in vivo and indicate that pulsed-SILAM based approaches can identify specific anatomical sites and biological pathways likely to be suitable for drug targeting in neurodegenerative disorders.
HostingRepository	PRIDE
AnnounceDate	2020-06-23
AnnouncementXML	Submission_2020-06-23_01:52:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ser Sue Ng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-15 03:00:27	ID requested
⏵ 1	2020-06-23 01:52:10	announced
2	2024-10-22 05:07:45	announced	2024-10-22: Updated project metadata.

Ng SS, Park JE, Meng W, Chen CP, Kalaria RN, McCarthy NE, Sze SK, Pulsed SILAM Reveals In Vivo Dynamics of Murine Brain Protein Translation. ACS Omega, 5(23):13528-13540(2020) [pubmed]

curator keyword: Biological

submitter keyword: pulsed SILAM, actively translating protein, pSIVOM, brain physiology

Siu Kwan Sze
contact affiliation	1School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore
contact email	sksze@ntu.edu.sg
lab head
Ser Sue Ng
contact affiliation	Newman Siu Kwan SZE's Lab, School of Biological Science, Nanyang Technological University
contact email	ngsersue@ntu.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD013502

PRIDE project URI

• PRIDE
  1. PXD013502
     1. Label: PRIDE project
     2. Name: In vivo murine brain protein synthesis uncover the molecular physiological process in brain