PXD013450-2

PXD013450 is an original dataset announced via ProteomeXchange.

Title	Circadian Clock Regulation of the Secretory Pathway
Description	Proteins destined for secretion move from the endoplasmic reticulum (ER, the site of synthesis) to Golgi cisternae then onto the cell surface in transport vesicles. Although the mechanism of anterograde and retrograde transport via vesicles is well understood the temporal coordination of transport between organelles has not been studied. Here we show that the extracellular levels of collagen-I (the most abundant protein in vertebrates) are 24-h rhythmic in tendon, which is the richest source of collagen-I. Rhythmicity is the result of circadian clock control of the secretory pathway via ER-ribosome docking, Tango1-dependent ER export, phosphodiesterase-dependent Golgi-ER retrograde transport of Hsp47 (a collagen molecular chaperone), and Vps33b-dependent post Golgi export, which pause collagen-I transport at each node in the pathway during a 24-hour cycle. The structure and mechanical properties of tendon are also 24-hourly rhythmic. Thus, the circadian clock is a master logistic operator of the secretory pathway in mammalian cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:03.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Venkatesh Mallikarjun
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-10 08:45:43	ID requested
1	2019-10-28 12:08:53	announced
⏵ 2	2024-10-22 04:55:03	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: mouse,tendon,Collagen,Protein trafficking

Karl Kadler
contact affiliation	Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT UK
contact email	karl.kadler@manchester.ac.uk
lab head
Venkatesh Mallikarjun
contact affiliation	University of Manchester
contact email	venkatesh.mallikarjun@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD013450

PRIDE project URI

• PRIDE
  1. PXD013450
     1. Label: PRIDE project
     2. Name: Circadian Clock Regulation of the Secretory Pathway