PXD013408-1

PXD013408 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of induced and inhibited early Hh signaling in human medulloblastoma cells
Description	Aberrant sonic hedeghog signaling is implicated in the development of various cancer entities such as medulloblastoma. The canonical signaling cascade has been studied for years. Activation of GLI transcription factors was revealed as the driving force upon pathway activation. Phosphorylation by Proteinkinase A, Casein Kinase 1 and Glycogen Synthase Kinase 3 β has been found to influence the degradation of the GLI transcription factors. However, the deeper role of phosphorylation in the signal transduction remains unclear. We, therefore, applied comprehensive HPLC-MS/MS based phosphoproteomics to reveal phosphorylation dynamics underlying the chemical activation and inhibition of sonic hedgehog signaling in human medulloblastoma cells. Human medulloblastoma cells were treated with SAG (Hh pathway induction) and Vismodegib (Hh pathway inhibition) for 5 and 15 minutes. Our phosphoproteomic profiling revealed a central role of phosphorylation in the regulation of ciliary assembly, trafficking and signal transduction after 5 minutes treatment. ERK/MAPK signaling besides protein kinase A signaling and mTOR signaling were differentially regulated. Activation of Polo-like kinase 1 and inhibtion of Caseinkinase 2A1 was characteristic for Vismodegib treatment while SAG treatment induced Aurora kinase A activity. Distinctive phosphorylation of central players of sonic Hh signaling such as Smoothened, SUFU, Gli2 and Gli3 was obtained after 15 minutes treatment.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_11:14:46.945.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamara Scheidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-08 08:14:57	ID requested
⏵ 1	2021-09-08 11:14:47	announced
2	2024-10-22 05:26:18	announced	2024-10-22: Updated project metadata.

Scheidt T, Alka O, Gonczarowska-Jorge H, Gruber W, Rathje F, Dell'Aica M, Rurik M, Kohlbacher O, Zahedi RP, Aberger F, Huber CG, Phosphoproteomics of short-term hedgehog signaling in human medulloblastoma cells. Cell Commun Signal, 18(1):99(2020) [pubmed]

submitter keyword: Hedgehog signaling, Phosphorylation, Phosphoproteomics, Medulloblastoma, DAOY cells

Christian G. Huber
contact affiliation	Biosciences, Bioanalytical Research Labs, University of Salzburg, Salzburg, Austria
contact email	c.huber@sbg.ac.at
lab head
Tamara Scheidt
contact affiliation	PhD Student
contact email	tamara.scheidt@sbg.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013408
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of induced and inhibited early Hh signaling in human medulloblastoma cells