PXD013401-2

PXD013401 is an original dataset announced via ProteomeXchange.

Title	Functions of the FtsH1/3 complex in the nutrient stress response of Synechocystis
Description	FtsH zinc metalloproteinases are membrane-intrinsic and occur in bacteria, chloroplasts, and mitochondria. They are involved in diverse cellular processes including protein quality control and regulation. The cyanobacterium Synechocystis possesses four FtsH homologs, designated FtsH1-4 which function as both homo- and hetero-oligomeric complexes. For example, the FtsH1/3 complex is known to have a critical role in iron homeostasis. This project is aimed at the investigation of the involvement of the FtsH1/3 complex in cyanobacterial responses to nitrogen, phosphate and inorganic carbon starvation. By proteomic comparisons of the effects of these nutrient stresses in WT and an FtsH1-down mutant, we demonstrate significant differences in the abundances of transcription factors that complement the responses to nutrient stress at the transcriptome level. We propose a mechanism of epistatic regulation of stress-response gene expression by FtsH1/3 complex-mediated degradation of suppressor transcription factors.
HostingRepository	PRIDE
AnnounceDate	2021-08-16
AnnouncementXML	Submission_2021-08-15_23:58:30.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-08 07:01:25	ID requested
1	2021-08-13 05:33:19	announced
⏵ 2	2021-08-15 23:58:31	announced	2021-08-16: Updated project metadata.
3	2024-10-22 05:24:34	announced	2024-10-22: Updated project metadata.

Krynick, á V, Georg J, Jackson PJ, Dickman MJ, Hunter CN, Futschik ME, Hess WR, Komenda J, sp strain PCC 6803. Plant Cell, 31(12):2912-2928(2019) [pubmed]

10.1105/TPC.19.00411;

submitter keyword: nutrient stress, FtsH,Synechocystis, transcription factors

Joseph Komenda
contact affiliation	Centre Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic, 6 Novohradská 237, Třeboň, 379 81, Czech Republic
contact email	komenda@alga.cz
lab head
Philip Jackson
contact affiliation	Department of Molecular Biology and Biotechnology, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD013401

PRIDE project URI

• PRIDE
  1. PXD013401
     1. Label: PRIDE project
     2. Name: Functions of the FtsH1/3 complex in the nutrient stress response of Synechocystis