PXD013375 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | 2D Spatial Proteomic Mapping Shows Distinct Regional Differences in the Bovine Cardiac Proteome |
Description | This study highlights that organs can have variable sub-proteomes that do not necessarily correspond to anatomical features. The bovine heart as a model demonstrates at least 3-5 separate proteomes exist within a 2D plane of the heart. The main differences between these proteomes were the biochemical pathways utilized energy substrates. By mapping individual protein expression across a 2D image we can see where proteins of interest such as disease associated proteins are specifically expressed and how specific areas of the heart have subtle but significant differences in the ratio of mitochondrial complexes. This may provide an idea of where to focus analysis on in the cardiac proteome for future studies and into the different mechanisms associated with function, stresses and potential disease processes We set out to fully proteomically characterise a whole section of heart to see if the degree of proteome changes with very different anatomical and physiological function and which biochemical pathways are more prevalent or important in different areas and whether this could be explained by the physiological requirement of that area of the heart. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_06:27:03.733.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD013375 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Wendy Heywood |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | Ammonia-loss; Dehydrated; Oxidation; Carbamidomethyl |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-04 07:05:49 | ID requested | |
⏵ 1 | 2024-10-17 06:27:04 | announced | |
Publication List
10.3390/life14080970; |
10.6019/PXD013375; |
Heywood WE, Searle J, Collis R, Doykov I, Ashworth M, Sebire N, Bamber A, Gautel M, Eaton S, Coats CJ, Elliott PM, Mills K, A Proof of Principle 2D Spatial Proteome Mapping Analysis Reveals Distinct Regional Differences in the Cardiac Proteome. Life (Basel), 14(8):(2024) [pubmed] |
Keyword List
submitter keyword: Heart, desmoglein 2, proteomics, bovine, mitochondria, proteome |
Contact List
Dr Kevin Mills |
contact affiliation | UCL Centre for Inborn Errors of Metabolism Institute of Child Health at Great Ormond Street Hospital University College London United Kingdom |
contact email | kevin.mills@ucl.ac.uk |
lab head | |
Wendy Heywood |
contact affiliation | University College London |
contact email | wendy.heywood@ucl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD013375 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013375
- Label: PRIDE project
- Name: 2D Spatial Proteomic Mapping Shows Distinct Regional Differences in the Bovine Cardiac Proteome