PXD013375-1

PXD013375 is an original dataset announced via ProteomeXchange.

Title	2D Spatial Proteomic Mapping Shows Distinct Regional Differences in the Bovine Cardiac Proteome
Description	This study highlights that organs can have variable sub-proteomes that do not necessarily correspond to anatomical features. The bovine heart as a model demonstrates at least 3-5 separate proteomes exist within a 2D plane of the heart. The main differences between these proteomes were the biochemical pathways utilized energy substrates. By mapping individual protein expression across a 2D image we can see where proteins of interest such as disease associated proteins are specifically expressed and how specific areas of the heart have subtle but significant differences in the ratio of mitochondrial complexes. This may provide an idea of where to focus analysis on in the cardiac proteome for future studies and into the different mechanisms associated with function, stresses and potential disease processes We set out to fully proteomically characterise a whole section of heart to see if the degree of proteome changes with very different anatomical and physiological function and which biochemical pathways are more prevalent or important in different areas and whether this could be explained by the physiological requirement of that area of the heart.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_06:27:03.733.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013375
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wendy Heywood
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	Ammonia-loss; Dehydrated; Oxidation; Carbamidomethyl
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-04 07:05:49	ID requested
⏵ 1	2024-10-17 06:27:04	announced

10.3390/life14080970;

10.6019/PXD013375;

Heywood WE, Searle J, Collis R, Doykov I, Ashworth M, Sebire N, Bamber A, Gautel M, Eaton S, Coats CJ, Elliott PM, Mills K, A Proof of Principle 2D Spatial Proteome Mapping Analysis Reveals Distinct Regional Differences in the Cardiac Proteome. Life (Basel), 14(8):(2024) [pubmed]

submitter keyword: Heart, desmoglein 2, proteomics, bovine, mitochondria, proteome

Dr Kevin Mills
contact affiliation	UCL Centre for Inborn Errors of Metabolism Institute of Child Health at Great Ormond Street Hospital University College London United Kingdom
contact email	kevin.mills@ucl.ac.uk
lab head
Wendy Heywood
contact affiliation	University College London
contact email	wendy.heywood@ucl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013375
     1. Label: PRIDE project
     2. Name: 2D Spatial Proteomic Mapping Shows Distinct Regional Differences in the Bovine Cardiac Proteome