PXD013359 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of several non pathogenic mutants from Botrytis cinerea. |
Description | The grey mould fungus Botrytis cinerea is a typical necrotrophic fungus that can infect hundreds of host plants, including high-value crops such as grapevine, strawberry and tomato. In order to find new important genes involved in the fungal virulence, a mutant library was generated by random insertional mutagenesis, using Agrobacterium tumefaciens mediated transformation. Ten mutants exhibiting total loss of virulence towards different host plants were considered. Their molecular characterization revealed a single T-DNA insertion in unique and different loci. Using a proteomics approach, the secretome of four of these strains was compared to that of the parental strain and a common profile of reduced lytic enzymes was recorded. Significant variations in this profile, notably proteases and hemicellulases deficiencies, were observed and validated by biochemical tests. They were also a hallmark of the remaining six non pathogenic strains, suggesting the importance of these secreted proteins in the infectious process. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-03 |
AnnouncementXML | Submission_2021-11-03_02:51:34.279.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dupuy Jean-William |
SpeciesList | scientific name: Botrytis cinerea; NCBI TaxID: 40559; |
ModificationList | acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-03 06:57:09 | ID requested | |
⏵ 1 | 2021-11-03 02:51:35 | announced | |
Publication List
de Vall, é, e A, Bally P, Bruel C, Chandat L, Choquer M, Dieryckx C, Dupuy JW, Kaiser S, Latorse MP, Loisel E, Mey G, Morgant G, Rascle C, Schumacher J, Simon A, Souibgui E, Viaud M, Villalba F, Poussereau N, ATMT-Mutants? Front Microbiol, 10():2829(2019) [pubmed] |
Keyword List
submitter keyword: Botrytis cinerea, non phytopathogenic mutant, quantitative proteome |
Contact List
Nathalie Poussereau |
contact affiliation | Equipe de génomique fonctionnelle des champignons pathogènes des plantes. UMR 5240 Microbiologie Adaptation et Microbiologie. 14 impasse Pierre Baizet 69263 Lyon , Cedex 09. France |
contact email | nathalie.poussereau@univ-lyon1.fr |
lab head | |
Dupuy Jean-William |
contact affiliation | Plateforme Proteome Bordeaux |
contact email | jean-william.dupuy@u-bordeaux.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013359
- Label: PRIDE project
- Name: Proteomic analysis of several non pathogenic mutants from Botrytis cinerea.