PXD013310 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic analysis of simian primary hepatocytes reveals candidate molecular markers for permissiveness to relapsing malaria Plasmodium cynomolgi |
Description | A major obstacle in deciphering the hepatic stage of the malaria parasite has been the challenges associated with culturing the infected hepatocytes through the entire liver stage cycle, including that of the dormant form known as hypnozoites. Primary hepatocytes lose their specialized functions in long-term in vitro culture. Hepatocyte infection represents the first step for clinically silent infection and development of malaria parasite Plasmodium in the liver. Thus this liver stage is an ideal target for development of novel antimalarial drugs and vaccine. However, drug discovery against Plasmodium liver stage is severely hampered by the poor understanding of host-cell and parasites interactions during the liver stage infection and development. In this study, we have performed tandem mass tags (TMT) labelling based quantitative proteomic analysis in simian primary hepatocytes cultured in three different systems of susceptibility to plasmodium infection. Our results represent the first documentation of potentially essential molecular markers including asialoglycoprotein receptor (ASGPR), apolipoproteins, squalene synthase and scavenger receptor B1 (SR-BI) required for productive infection and full development in relapsing Plasmodium species. The identification of these candidate proteins for constructive infection and development of Plasmodium in malaria paves the way to explore them as therapeutic targets. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-02 |
AnnouncementXML | Submission_2019-09-02_01:04:25.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bamaprasad Dutta |
SpeciesList | scientific name: Macaca fascicularis; NCBI TaxID: 9541; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-29 06:52:31 | ID requested | |
⏵ 1 | 2019-09-02 01:04:26 | announced | |
Publication List
Dembele L, Gupta DK, Dutta B, Chua ACY, Sze SK, Bifani P, cynomolgi. Proteomics, 19(19):e1900021(2019) [pubmed] |
Keyword List
submitter keyword: Quantitative proteomic, LC-MS/MS, TMT, Malaria liver stage, Drug assay model development, Biomarker, permissiveness to infection |
Contact List
Siu Kwan SZE |
contact affiliation | School of Biological Sciences Nanyang Technological University 60 Nanyang Drive, Singapore 637551 |
contact email | sksze@ntu.edu.sg |
lab head | |
Bamaprasad Dutta |
contact affiliation | Nanyang Technological University |
contact email | bama0001@e.ntu.edu.sg |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013310
- Label: PRIDE project
- Name: Quantitative proteomic analysis of simian primary hepatocytes reveals candidate molecular markers for permissiveness to relapsing malaria Plasmodium cynomolgi