PXD013304 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | VEX1-GFP Pull-downs |
Description | We affinity-purified VEX1GFP-associated proteins using cryomilling and high-affinity nanobodies. The procedure was carried out initially in insect-stage T. brucei, for which protocols were established, and then in bloodstream-form T. brucei. Quantitative proteomic analysis revealed tag-dependent enrichment of GFP and the same set of five proteins in four independent experiments; VEX1 was enriched, as expected, but also Tb927.11.13380, an ortholog of the nonsense-mediated mRNA-decay (NMD) ATP-dependent superfamily 1-type helicases, UPF1/SMG2/NAM7/Rent1 19), now designated VEX2 (predicted 224-kDa, 2026 residues). The other three proteins that displayed tag-dependent enrichment were all three components of chromatin assembly factor 1 (CAF-1; CAF-1a, Tb927.8.3980; CAF-1b, Tb927.10.7050, CAF-1c, Tb927.11.4970a), the evolutionary conserved hetero-trimeric replication-associated histone chaperone. |
HostingRepository | PRIDE |
AnnounceDate | 2019-05-31 |
AnnouncementXML | Submission_2019-05-31_03:22:00.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD013304 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Joana Faria |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | Oxidation; Dioxidation; Carbamidomethyl; Acetyl; Gln->pyro-Glu |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-29 04:53:38 | ID requested | |
⏵ 1 | 2019-05-31 03:22:01 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: VEX1 |
VSG |
allelic exclusion |
Trypanosoma brucei |
Contact List
David Horn |
contact affiliation | Professor of Parasite Molecular Biology Biological Chemistry and Drug Discovery Wellcome Centre for Anti-Infectives Research (WCAIR) School of Life Sciences, University of Dundee |
contact email | d.horn@dundee.ac.uk |
lab head | |
Joana Faria |
contact affiliation | Wellcome Trust Centre for Anti-infectives Research, School of Life Sciences, University of Dundee |
contact email | jrcorreiafaria@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013304
- Label: PRIDE project
- Name: VEX1-GFP Pull-downs