PXD013296 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins |
Description | Background: The human genome consists of considerable portions derived from retroviruses inherited for millions of years. So-called human endogenous retroviruses (HERVs) are usually severely mutated, yet some coding-competent HERVs exist. The HERV-K(HML-2) group includes evolutionarily young proviruses that still encode typical retroviral proteins. HERV-K(HML-2) has been implicated in various human diseases because transcription is often upregulated and some of the encoded proteins are known to affect cell biology. HERV-K(HML-2) Protease (Pro) has received little attention so far, although it appears expressed in some disease contexts and other retroviral proteases are known to process cellular proteins. Results: We set out to identify human cellular proteins being substrates of HERV-K(HML-2) Pro employing a modified Terminal Amine Isotopic Labeling of Substrates (TAILS) procedure. Thousands of human proteins were identified as significantly processed by HERV-K(HML-2) Pro. Identified proteins locate to various cellular compartments and participate in diverse, often disease-relevant cellular processes. We verified cleavage of a majority of selected human proteins in vitro and in vivo. Conclusions: Hundreds, if not thousands of cellular proteins are potential substrates of HERV-K(HML-2) Pro. It is conceivable that even low-level expression of HERV-K(HML-2) Pro has a functional impact on cell biology and thus relevance for human diseases. Specific studies will be required to elucidate effects of HERV-K(HML-2) Pro expression regarding human substrate proteins, cell biology and disease. Endogenous retrovirus-encoded Pro activity may also be relevant for disease development in species other than human. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-02 |
AnnouncementXML | Submission_2019-09-02_00:46:06.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Oliver Schilling |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-29 01:28:12 | ID requested | |
⏵ 1 | 2019-09-02 00:46:07 | announced | |
Publication List
Rigogliuso G, Biniossek ML, Goodier JL, Mayer B, Pereira GC, Schilling O, Meese E, Mayer J, A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins. Mob DNA, 10():36(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Proteolysis, retrovirus |
Contact List
Oliver Schilling |
contact affiliation | Institute of Surgical Pathology Faculty of Medicine - University of Freiburg |
contact email | oliver.schilling@mol-med.uni-freiburg.de |
lab head | |
Oliver Schilling |
contact affiliation | University of Freiburg |
contact email | oliver.schilling@mol-med.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD013296
- Label: PRIDE project
- Name: A human endogenous retrovirus encoded protease potentially cleaves numerous cellular proteins