PXD013232 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cellular concentrations of Protein Kinase D1 in HEK293T and INS-1 cells |
Description | Protein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo concentration dependent dimerization in vitro. To test whether the cellular concentration of PKD1 is in the range of the dissociation constant obtained, the expression level of PKD1 was investigated by PRM. To this end, an equimolar mixture of pure, recombinant protein comprising the PKD N-terminus (residues 48-198) and C-terminus (residues 395-892) was used to spike the cell lysates of human HEK293T cells or the rat cell line INS-1. In the latter case, only peptides that are identical between the human and rat PKD1 protein were used to estimate the cellular expression level of PKD1. |
HostingRepository | PRIDE |
AnnounceDate | 2019-08-19 |
AnnouncementXML | Submission_2019-08-19_00:11:33.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Markus Hartl |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos; Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-26 00:58:51 | ID requested | |
⏵ 1 | 2019-08-19 00:11:34 | announced | |
Publication List
Elsner DJ, Siess KM, Gossenreiter T, Hartl M, Leonard TA, A ubiquitin-like domain controls protein kinase D dimerization and activation by trans-autophosphorylation. J Biol Chem, 294(39):14422-14441(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: protein kinase D, PKD1, HEK293T, INS-1 |
Contact List
Thomas A. Leonard |
contact affiliation | Department of Structural and Computational Biology, Max F. Perutz Laboratories (MFPL), Campus Vienna Biocenter 5, 1030 Vienna, Austria |
contact email | thomas.leonard@meduniwien.ac.at |
lab head | |
Markus Hartl |
contact affiliation | Mass Spectrometry Facility, Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 3, 1030 Vienna |
contact email | markus.hartl@univie.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD013232 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013232
- Label: PRIDE project
- Name: Cellular concentrations of Protein Kinase D1 in HEK293T and INS-1 cells