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PXD013215-1

PXD013215 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAPEX2-mediated proximity labeling of mitochondrial matrix and chromatin in budding yeast
DescriptionEnzyme-catalyzed proximity labeling (PL) with biotin is a novel approach to map organelle compartmentalization and protein interaction networks in living cells. Here, we present a new method based on semi-permeabilized yeast cells and the enzyme APEX2, an engineered ascorbate peroxidase. Combined with stable isotope labeling by amino acids in cell culture (SILAC), we demonstrate proteomic mapping of a membrane-enclosed and a semi-open compartment, the mitochondrial matrix and the nucleus. APEX2 PL revealed nuclear proteins that were previously not identified by conventional techniques. One of these, the Yer156C protein, is highly conserved but of unknown function. In follow-up experiments using quantitative PL with Yer156C-APEX2 we discovered an array of Yer156C interactors. Thus, our approach establishes APEX2 PL as an effective and versatile tool that complements the powerful methods palette for the model system yeast.
HostingRepositoryPRIDE
AnnounceDate2019-07-17
AnnouncementXMLSubmission_2019-07-17_00:15:51.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNicolas Nalpas
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-03-25 06:24:15ID requested
12019-07-17 00:15:52announced
22024-10-22 04:03:04announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Mitochondrial matrix, chromatin, budding yeast Yer156C interactome
Contact List
Dr. Birgit Singer-Krüger
contact affiliationInterfaculty Institute of Biochemistry Universität Tübingen Hoppe-Seyler-Straße 4 72076 Tübingen Germany
contact emailbirgit.singer-krueger@uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliationTuebingen University
contact emailnalpas.nicolas@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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