PXD013140 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Glycosylation Design Space for Recombinant Lysosomal Replacement Enzymes Produced in CHO Cells |
Description | Lysosomal storage diseases are rare life-threatening disorders caused by deficiency of a lysosomal enzyme, and delivery of a recombinant replacement enzyme is the primary therapy for several of these diseases. The structures of N-glycans on recombinant replacement enzymes are important for their therapeutic efficacy, biodistribution and circulation time. Here we use alpha-galactosidase A (GLA) and beta-glucoscerebrosidase (GBA) as representative replacement enzymes, and present a panel of genetically engineered CHO cells that enable production of lysosomal replacement enzymes with different N-glycan features. We also tested several GLA variants with distinct glycoforms in a Fabry mouse model and present evidence that GLA glycoforms capped with alpha 2-3 linked sialic acids may represent a new strategy to overcome the most critical problems of rapid clearance in liver and poor biodistribution found with current ERTs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:06:34.996.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sergey Vakhrushev |
SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | complex glycosylation |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-19 03:10:53 | ID requested | |
1 | 2019-05-31 06:02:14 | announced | |
⏵ 2 | 2024-10-22 04:06:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41467-019-09809-3; |
Tian W, Ye Z, Wang S, Schulz MA, Van Coillie J, Sun L, Chen YH, Narimatsu Y, Hansen L, Kristensen C, Mandel U, Bennett EP, Jabbarzadeh-Tabrizi S, Schiffmann R, Shen JS, Vakhrushev SY, Clausen H, Yang Z, The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells. Nat Commun, 10(1):1785(2019) [pubmed] |
Keyword List
submitter keyword: N-glycoproteomics, enzyme replacement therapy,Glycoengineering |
Contact List
Henrik Clausen |
contact affiliation | Copenhagen Center for Glycomics (CCG), Department of Cellular and Molecular Medicine, University of Copenhagen |
contact email | hclau@sund.ku.dk |
lab head | |
Sergey Vakhrushev |
contact affiliation | Department of Cellular and Molecular Medicine |
contact email | seva@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013140
- Label: PRIDE project
- Name: The Glycosylation Design Space for Recombinant Lysosomal Replacement Enzymes Produced in CHO Cells