PXD013074-1

PXD013074 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization and label-free comparison of outer membrane vesicles from Francisella tularensis subsp. holarctica strain FSC200 isolated from different cultivation conditions
Description	Francisella tularensis is a Gram-negative, facultative intracellular bacterium, causing a severe disease known as tularemia. It is known to secrete unusually shaped nanotubular outer membrane vesicles (OMV) loaded with number of virulence factors and immunoreactive proteins. In this study the nanotubes were purified from a clinical isolate of subsp. holarctica strain FSC200. We here provide a comprehensive proteomic characterization of OMV isolated from bacteria grown under different cultivation conditions simulating the diverse conditions of F. tularensis life cycle. These included conditions mimicking the environment inside the mammalian host during inflammation: oxidative stress, low pH and high temperature (42 °C); and by contrast, low temperature (25 °C) that mimicked the external milieu and agar plate cultivation. We observed several-fold increase in vesiculation rate at high temperature and low pH but the differences were not only in the amount of secreted OMV but particularly in their protein cargo. Amongst the proteins preferentially selectively packed into OMV under conditions mimicking mammalian host many were previously described as virulence factors connected to the unique intracellular trafficking of Francisella. Protein changes revealed also high probability of alterations of the bacterial surface on the level of lipopolysaccharide, polysaccharide capsule and phospholipids.
HostingRepository	PRIDE
AnnounceDate	2019-09-30
AnnouncementXML	Submission_2019-09-30_02:36:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Klimentova
SpeciesList	scientific name: Francisella tularensis subsp. holarctica FSC200; NCBI TaxID: 351581; scientific name: Francisella tularensis subsp. holarctica; NCBI TaxID: 119857;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-13 03:46:06	ID requested
⏵ 1	2019-09-30 02:36:22	announced
2	2019-11-12 02:08:13	announced	2019-11-12: Updated publication reference for PubMed record(s): 31649645.
3	2024-10-22 04:10:46	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Francisella tularensis

FSC200

outer membrane vesicles

stress response

Jiri Stulik
contact affiliation	Department of Molecular Pathology, Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
contact email	jiri.stulik@unob.cz
lab head
Jana Klimentova
contact affiliation	Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
contact email	jana.klimentova@unob.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013074
     1. Label: PRIDE project
     2. Name: Proteomic characterization and label-free comparison of outer membrane vesicles from Francisella tularensis subsp. holarctica strain FSC200 isolated from different cultivation conditions