PXD013055 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion |
Description | Cell-cell communication in multicellular organisms depends on the dynamic and reversible phosphorylation of protein tyrosine residues. The receptor-linked protein tyrosine phosphatases (RPTPs) receive cues from the extracellular environment and are well placed to influence cell signaling. However, the direct events downstream of these receptors have been challenging to resolve. We report here that the homophilic receptor PTPRK is stabilized at cell-cell contacts in epithelial cells. By combining interaction studies, quantitative tyrosine phosphoproteomics, proximity labelling and dephosphorylation assays we identify high confidence PTPRK substrates. PTPRK directly and selectively dephosphorylates at least five substrates, including Afadin, PARD3 and -catenin family members, which are all important cellcell adhesion regulators. In line with this, loss of PTPRK phosphatase activity leads to disrupted cell junctions and increased invasive characteristics. Thus, identifying PTPRK substrates provides insight into its downstream signaling and a potential molecular explanation for its proposed tumor suppressor function. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-29 |
AnnouncementXML | Submission_2019-04-02_04:13:12.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jack Houghton |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-12 02:49:12 | ID requested | |
1 | 2019-03-29 11:24:22 | announced | |
⏵ 2 | 2019-04-02 04:13:13 | announced | Updated publication reference for PubMed record(s): 30924770. |
Publication List
Fearnley GW, Young KA, Edgar JR, Antrobus R, Hay IM, Liang WC, Martinez-Martin N, Lin W, Deane JE, Sharpe HJ, The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion. Elife, 8():(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Phosphorylation |
SILAC |
Contact List
Dr Hayley J Sharpe |
contact affiliation | Cambridge Institute for Medical Research |
contact email | hjs49@cam.ac.uk |
lab head | |
Jack Houghton |
contact affiliation | University of Cambridge |
contact email | jwh65@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013055
- Label: PRIDE project
- Name: The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion