PXD013034 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Epstein-Barr Virus Induced One-Carbon Metabolism Drives B-Cell Transformation |
Description | Epstein-Barr virus (EBV) causes Burkitt, Hodgkin, and post-transplant B-cell lymphomas. How EBV remodels metabolic pathways to support rapid B-cell outgrowth remains largely unknown. To gain insights, primary human B-cells were profiled by tandem-mass-tag based proteomics at rest and at 9 time points after infection. >8000 host and 29 viral proteins were quantified, revealing mitochondrial remodeling and induction of one-carbon (1C) metabolism. EBV-encoded EBNA2 and its target MYC were required for upregulation of the central mitochondrial 1C enzyme MTHFD2, which played key roles in EBV-driven B-cell growth and survival. MTHFD2 was critical for maintaining elevated NADPH levels in infected cells, and oxidation of mitochondrial NADPH diminished B-cell proliferation. Tracing studies underscored contributions of 1C to nucleotide synthesis, NADPH production and redox defense. EBV upregulated import and synthesis of serine to augment 1C flux. Our results highlight EBV-induced 1C as a potential therapeutic target and provide a new paradigm for viral onco-metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2019-06-28 |
AnnouncementXML | Submission_2019-06-28_03:16:16.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Weekes |
SpeciesList | scientific name: Epstein-barr virus strain p3hr-1; NCBI TaxID: 82829; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; carbamoylated residue; monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-11 02:40:22 | ID requested | |
⏵ 1 | 2019-06-28 03:16:17 | announced | |
2 | 2019-07-04 04:57:45 | announced | Updated publication reference for PubMed record(s): 31257153. |
3 | 2024-10-22 04:11:24 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: Epstein-Barr virus, herpesvirus, quantitative proteomics, tandem mass tag, host-pathogen interaction, one-carbon metabolism |
Contact List
Michael Weekes |
contact affiliation | Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 0XY |
contact email | Mpw1001@cam.ac.uk |
lab head | |
Michael Weekes |
contact affiliation | University of Cambridge |
contact email | mpw1001@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013034
- Label: PRIDE project
- Name: Epstein-Barr Virus Induced One-Carbon Metabolism Drives B-Cell Transformation