PXD012967 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bacteriocins in Lactobacillus acidophlius membrane vesicles |
Description | Recent reports have shown that Gram-positive bacteria actively secrete spherical nanometer-sized proteolipid membrane vesicles (MVs) into their surroundings. Though MVs have been implicated in a broad range of biological functions, few studies have been conducted to examine their potential as delivery vehicles of antimicrobials. Here, we investigate the natural ability of Lactobacillus acidophilus MVs to carry and deliver bacteriocin peptides to the opportunistic pathogen, Lactobacillus delbrueckii. We demonstrate that upon treatment with lactacin B-inducing peptide the proteome of the secreted MVs is enriched in putative bacteriocins encoded by the lab operon. Further, we show that purified MVs inhibit growth and form membrane pores in L. delbrueckii, which is confirmed using a number of microscopy techniques and spectrophotometry. These results show that L. acidophilus MVs serve as conduits for antimicrobials to competing cells in the environment, suggesting a potential role for MVs in complex communities such as the gut microbiome. With the potential for controlling their payload through microbial engineering, MVs produced by L. acidophilus may be an interesting platform for effecting change in complex microbial communities or aiding in the development of new biomedical therapeutics. |
HostingRepository | PRIDE |
AnnounceDate | 2020-03-20 |
AnnouncementXML | Submission_2020-03-20_00:44:18.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD012967 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Scott Dean |
SpeciesList | scientific name: Lactobacillus acidophilus NCFM; NCBI TaxID: 272621; |
ModificationList | Deamidated; Oxidation |
Instrument | TripleTOF 5600; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-03-05 03:56:49 | ID requested | |
⏵ 1 | 2020-03-20 00:44:19 | announced | |
2 | 2020-06-12 03:49:27 | announced | 2020-06-12: Updated publication reference for PubMed record(s): 32425905. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Lactobacillus, membrane vesicles, targeted proteomics |
Contact List
Scott Walper |
contact affiliation | Naval Research Laboratory |
contact email | scott.walper@nrl.navy.mil |
lab head | |
Scott Dean |
contact affiliation | Naval Research Laboratory |
contact email | scottnathandean@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012967
- Label: PRIDE project
- Name: Bacteriocins in Lactobacillus acidophlius membrane vesicles