PXD012948-2

PXD012948 is an original dataset announced via ProteomeXchange.

Title	Primary human MSCs treated with two different RNAi against SUN2
Description	Cells resident in tissues must be resilient to the physical demands of their surroundings. Our current understanding of cellular mechano-signalling is largely based on static systems, but these models do not reproduce the dynamic nature of living tissue. Here, we examined the time-resolved response of primary human mesenchymal stem cells (hMSCs) to periods of cyclic tensile strain (CTS). We observed parallels between morphological changes following low-intensity strain (1 hour, 4% CTS at 1 Hz) and responses to increased substrate stiffness. However, as the strain regime was intensified (CTS at ≥ 2 Hz), we characterised a broad, structured and reversible protein-level response, even as transcription was apparently shut down. Regulation of the linker of nucleo- and cytoskeleton (LINC) complex proteins, and specifically of SUN domain-containing protein 2 (SUN2), was found to decouple mechano-transmission within the cell and hence isolate the nucleus from cellular deformation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:53:40.252.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Venkatesh Mallikarjun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-04 05:45:55	ID requested
1	2019-04-24 06:30:26	announced
⏵ 2	2024-10-22 04:53:47	announced	2024-10-22: Updated project metadata.

10.1038/s41467-019-11923-1;

Gilbert HTJ, Mallikarjun V, Dobre O, Jackson MR, Pedley R, Gilmore AP, Richardson SM, Swift J, Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading. Nat Commun, 10(1):4149(2019) [pubmed]

submitter keyword: SUN2, MSC, RNAi

Joe Swift
contact affiliation	Division of Cell Matrix Biology & Regenerative Medicine (L5) / Division of Cell Matrix Biology & Regenerative Medicine, University of Manchester
contact email	joe.swift@manchester.ac.uk
lab head
Venkatesh Mallikarjun
contact affiliation	University of Manchester
contact email	venkatesh.mallikarjun@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012948

PRIDE project URI

• PRIDE
  1. PXD012948
     1. Label: PRIDE project
     2. Name: Primary human MSCs treated with two different RNAi against SUN2