PXD012945-1

PXD012945 is an original dataset announced via ProteomeXchange.

Title	Free mRNA interactome upon heat shock in Hela cells
Description	The polypeptide-coding mRNAs are coated by specialized proteins at any stage of their lifecycle. One of few circumstances when free mRNA appears in the cytosol is the disassembly of polysomes during stress-induced shutdown of protein synthesis. Using quantitative mass spectrometry, we identified free RNA interactors in the heat-shocked mammalian cells and reconstituted the protein-RNA association in vitro. RNA-associated proteins displayed higher disorder and larger size, which supports the role of multivalent interactions during the initial phase of the RNA granule formation. Structural features of the free RNA interactors defined them as a subset of RNA-binding proteins. Our results reveal how free RNA can participate in reorganization of cellular functions during proteostasis stress.
HostingRepository	PRIDE
AnnounceDate	2019-06-28
AnnouncementXML	Submission_2019-06-28_05:29:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giulia Calloni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-04 01:56:18	ID requested
⏵ 1	2019-06-28 05:29:51	announced
2	2024-10-22 04:53:43	announced	2024-10-22: Updated project metadata.

Alriquet M, Mart, í, nez-Lim, ó, n A, Hanspach G, Hengesbach M, Tartaglia GG, Calloni G, Vabulas RM, Assembly of Proteins by Free RNA during the Early Phase of Proteostasis Stress. J Proteome Res, 18(7):2835-2847(2019) [pubmed]

curator keyword: Biological

submitter keyword: mRNA, proteostasis stress, RNA granules, stress granules

R. Martin Vabulas
contact affiliation	Buchmann Institute of Molecular Life Sciences and Institute of Biophysical Chemistry, Goethe University, Frankfurt am Main, Germany
contact email	vabulas@em.uni-frankfurt.de
lab head
Giulia Calloni
contact affiliation	Goethe University Frankfurt
contact email	calloni@em.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012945

PRIDE project URI

• PRIDE
  1. PXD012945
     1. Label: PRIDE project
     2. Name: Free mRNA interactome upon heat shock in Hela cells