PXD012942-2

PXD012942 is an original dataset announced via ProteomeXchange.

Title	N-glycoprotein capture mass spectrometry identifies candidate substrates of human Fut enzymes
Description	How disseminated tumor cells (DTCs) engage specific stromal components in distant organs for survival and outgrowth is a critical but poorly understood step of the metastatic cascade. Previous studies have demonstrated the importance of the epithelial-mesenchymal transition (EMT) in promoting the cancer stem cell properties needed for metastasis initiation, while the reverse process of mesenchymal-epithelial transition (MET) is required for metastatic outgrowth. Here we report that this paradoxical requirement for simultaneous induction of both MET and cancer stem cell traits in DTCs is provided by bone vascular niche E-selectin. Using cell surface alkoxyamine-biotinylation and label-free LC-MS/MS, Glg1 was identified as a top candidate Fut3/Fut6-dependent E-selectin ligand. We functionally validated their involvement in the formation of bone metastasis. These findings provide unique insights into the functional role of E-selectin as a component of the vascular niche criticalfor metastatic colonization in bone.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:53:55.079.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Todd Greco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-04 01:42:16	ID requested
1	2019-11-12 19:04:34	announced
⏵ 2	2024-10-22 04:54:03	announced	2024-10-22: Updated project metadata.

10.1038/s41556-019-0309-2;

Esposito M, Mondal N, Greco TM, Wei Y, Spadazzi C, Lin SC, Zheng H, Cheung C, Magnani JL, Lin SH, Cristea IM, Sackstein R, Kang Y, Bone vascular niche E-selectin induces mesenchymal-epithelial transition and Wnt activation in cancer cells to promote bone metastasis. Nat Cell Biol, 21(5):627-639(2019) [pubmed]

curator keyword: Biological

submitter keyword: PNGaseF, Fut,N-glycoprotein enrichment

Ileana Crisea
contact affiliation	Department of Molecular Biolgy, Princeton University, USA
contact email	icristea@princeton.edu
lab head
Todd Greco
contact affiliation	Molecular Biology, Princeton University
contact email	tgreco@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012942

PRIDE project URI

• PRIDE
  1. PXD012942
     1. Label: PRIDE project
     2. Name: N-glycoprotein capture mass spectrometry identifies candidate substrates of human Fut enzymes