PXD012920-4
PXD012920 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mass spectrometric analysis of accumulated TDP-43 in amyotrophic lateral sclerosis brains |
| Description | TDP-43 is the major disease-associated protein involved in the pathogenesis and progression of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitin-positive inclusions linked to TDP-43 pathology (FTLD-TDP). Abnormal phosphorylation, truncation and cytoplasmic mis-localization are known to be the characteristics for the aggregated forms of TDP-43, and gain of toxic abnormal TDP-43 or loss of function of physiological TDP-43 have been suggested as the cause of neurodegeneration. However, most of the post-translational modifications or truncation sites in the abnormal TDP-43 in brains of patients remain to be identified by protein chemical analysis. In this study, we carried out a highly sensitive liquid chromatography-mass spectrometry analysis of Sarkosyl-insoluble pathological TDP-43 from brains of ALS patients and identified several novel phosphorylation sites, deamidation sites, and cleavage sites. Almost all modifications were localized in the Gly-rich C-terminal half. Most of the cleavage sites identified in this study are novel and are located in N-terminal half, suggesting that these sites may be more accessible to proteolytic enzymes. The data obtained in this study provide a foundation for the molecular mechanisms of TDP-43 aggregation and ALS pathogenesis. |
| HostingRepository | jPOST |
| AnnounceDate | 2020-03-01 |
| AnnouncementXML | Submission_2022-09-18_02:19:17.090.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Fuyuki Kametani |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | unknown modification; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-02-28 23:20:40 | ID requested | |
| 1 | 2020-02-29 07:00:07 | announced | |
| 2 | 2020-03-19 00:45:06 | announced | 2020-03-19: Updated PubMed. |
| 3 | 2021-02-04 16:39:49 | announced | 2021-02-04: Updated FTP location. |
| ⏵ 4 | 2022-09-18 02:19:17 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Kametani F, Obi T, Shishido T, Akatsu H, Murayama S, Saito Y, Yoshida M, Hasegawa M, Mass spectrometric analysis of accumulated TDP-43 in amyotrophic lateral sclerosis brains. Sci Rep, 6():23281(2016) [pubmed] |
Keyword List
| submitter keyword: amyloid, TDP-43, inclusion body, phosphorylation, frontotemporal lobar degeneration with ubiquitin-positive inclusions, amyotrophic lateral sclerosis, proteomics, mass spectrometry |
Contact List
| Fuyuki Kametani | |
|---|---|
| lab head | |
| Fuyuki Kametani | |
| contact affiliation | Tokyo Metropolitan Institute of Medical Science |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000561/ |
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