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PXD012894-1

PXD012894 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSelective Binding of a Toxin and Phosphatidylinositides to a Mammalian Potassium Channel
DescriptionG-protein-gated inward rectifying potassium channels (GIRKs) require Gβγ subunits and phosphorylated phosphatidylinositides (PIPs) for gating. Although studies have provided insight into these interactions, the mechanism of how these events are modulated by Gβγ and the binding affinity between PIPs and GIRKs remains poorly understood. Here, native ion mobility mass spectrometry is employed to directly monitor small molecule binding events to mouse GIRK2. GIRK2 binds the toxin tertiapin Q and PIPs selectively and with significantly higher affinity than other phospholipids. A mutation in GIRK2 that causes a rotation in the cytoplasmic domain, similarly to Gβγ-binding to the wild-type channel, revealed differences in the selectivity towards PIPs More specifically, PIP isoforms known to weakly activate GIRKs have decreased binding affinity. Additionally, denaturing mass spectrometry and tryptic digest liquid chromatography mass spectrometry analysis was performed to confirm phosphorylation to GIRK2.
HostingRepositoryPRIDE
AnnounceDate2019-04-02
AnnouncementXMLSubmission_2019-04-02_07:52:59.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYang Liu
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-02-27 06:34:21ID requested
12019-04-02 07:53:00announced
22024-10-22 04:53:51announced2024-10-22: Updated project metadata.
Publication List
Liu Y, LoCaste CE, Liu W, Poltash ML, Russell DH, Laganowsky A, Selective binding of a toxin and phosphatidylinositides to a mammalian potassium channel. Nat Commun, 10(1):1352(2019) [pubmed]
Keyword List
submitter keyword: GIRK2, Potassium Channel, PIP2, Phosphorylation, Lipid Binding, Lipid Selectivity
Contact List
Arthur Laganowsky
contact affiliationTexas A&M University Department of Chemistry
contact emailalaganowsky@chem.tamu.edu
lab head
Yang Liu
contact affiliationTexas A&M University
contact emailyangalexliu@tamu.edu
dataset submitter
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Dataset FTP location
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